To evaluate the methodology for Discrimination of goatpoxvirus (GPV)and sheeppoxvirus (SPV),the p3genes and G 2protein2coupled chemokine receptor (GpCR) genes amplified from 3 strains of SPV and strains of GPV which isolated from the field samples were sequenced and compared with the corresponding sequences deposited in GenBank.Comparison of Hinf Ⅰrestriction enzyme sites of p3genes showed that there are sites located in 391 bp and 691 bp among all the SPV strains,and 1 site in 688 bp among GPV strains,respectively.Cladogram generated by sequence comparison with GpCR genes showed that the SPV and GPV belongs to the two different branches.The results indicated that the both genes,p3and GpCR,can be the candidates for development of methodologies for discrimination of GPV and SPV.
YAN Xin-min
,
ZHANG Qiang
,
WU Guo-hua
,
LI Jian
,
ZHU Hai-xia
. Comparative Analysis on the Methodologies for Discrimination between Goatpoxvirus and Sheeppoxvirus[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(6)
: 50
-53
.
DOI: 10.7668/hbnxb.2009.06.010
[1] 殷震,刘景华.动物病毒学[M].第2版.北京:科学出版社,1997:960-965.
[2] Rao T V,Bandyopadhyay S K.A.comprehensive review of goat pox and sheep pox and their diagnosis[J].Animal Health Research Review,2000,1(2):127-136.
[3] Altinel C.Studies on the blood chemistry of sheep infected with or vaccinated against sheep poxvirus[J].Pendik Veterinary Mikrobiyol-Derg,1993,24(2):203-129.
[4] Tulman E R,Afonso C L.The genomes of sheeppox and goatpox[J].Journal of Virology,2002,6(12):6054-6061.
[5] Carn V M,Kitching R P,Hammond J M,et al.Use of a recombinant antigen in an indirect ELISA for detecting bovine antibody to capripox virus[J].Journal Virology Methods,1994,49(2):285-294.
[6] Madhusudan H,Bimalendu M,Prabhakar A T,et al.Differentiation of sheep pox and goat pox by sequence analysis and PCR-RFLP of p32 gene[J].Virus Gene,2004,29(1):73-80.
[7] Bhanuprakash V,Indrani B K,Hosamani M,et al.The current status of sheep Pox disease[J].Comparative Immunology,Microbiology & Infectious Diseases,2006,29(2):27-60.
[8] Le Goff C,Lamien C E,Fakhfakh E,et al.Capripox G-protein-coupled chemokine receptor,a host-range gene suitable for virus-animal origin discrimination[J].Journal of General Virology,2009,4(1):32-26.
[9] Mondal B,Hosamani M,Dutta T K,et al.An outbreak of sheep pox on a sheep breeding fram in Jammu,India[J].Revue Science et Technique,2004,23(3):943-949.
[10] Black D N,Hammond J M,Kitching R P.Genomic relationship between capripox virus[J].Virus Research,1986,5:277-292.
