Abstract: To evaluate the methodology for Discrimination of goatpoxvirus (GPV)and sheeppoxvirus (SPV),the p3genes and G 2protein2coupled chemokine receptor (GpCR) genes amplified from 3 strains of SPV and strains of GPV which isolated from the field samples were sequenced and compared with the corresponding sequences deposited in GenBank.Comparison of Hinf Ⅰrestriction enzyme sites of p3genes showed that there are sites located in 391 bp and 691 bp among all the SPV strains,and 1 site in 688 bp among GPV strains,respectively.Cladogram generated by sequence comparison with GpCR genes showed that the SPV and GPV belongs to the two different branches.The results indicated that the both genes,p3and GpCR,can be the candidates for development of methodologies for discrimination of GPV and SPV.

Key words: p32 gene, GpCR gene, GPV, SPV, Discrimination

CLC Number: 

• S32