One of the sequenced 260 bp cDNAfragments was highly identified with auxin.The ESTwas extended using CAP3 software in the lab local net.Based on the extended sequence,a pair of primers was designed in the two side regions of its predicted sequence and a cDNA fragment of 608 bp was isolated from Mardler/ 7 3 Bainong 3217 using RTPCR method.The sequence encoded a peptide of 20deduced amino acid residues.The accession numbers were AY902381(gene) and (protein).The deduced molecular weight of protein was 23.0 kDa and the theoretical pI was 9.93.We used the Auxin response domain sequences identified from the auxin genes in plants as queries to search for auxin genes in both wheat and rice,and a polygenetic tree was constructed based on the corresponding auxin proteinsfromwheat and rice.The cell location and protein structure similarityof these genes were analyzed by searching the EST sequences of these genes,and it is essential to study this gene further based on the function of known similar genes.
Key words：
Wheat; Rice; Auxin
ZHANG Jun-hong
,
MENG Cheng-sheng
,
ZHANG Cai-ying
,
SHI Zheng
,
WANG Xiao-ying
,
LI Ai-li
,
MA Zhi-ying
. Bioinformatic Analysis of the auxin Gene Family in Wheat and Rice[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(6)
: 15
-19
.
DOI: 10.7668/hbnxb.2009.06.004
[1] http://www.biox.cn/content/20050703/22645.htm.
[2] 赵普庆,於维维,汪俏梅.生长素与其他信号之间的相互作用[J].植物生理学通讯,2004,40:246-250.
[3] 程鹏,蒋泓,欧阳琳,等.生物信息学技术分析并克隆超级稻生长素结合蛋白cDNA[J].农业现代化研究,2008,29(4):506-509.
[4] Chory J,Wu D.Weaving the complex web of signal transduction[J].Plant Physiol,2001,125:77-80.
[5] Tory Chhun1,Shin Taketa,Seiji Tsurumi,etal.Interaction between two auxin-resistant mutants and theireffects on lateral root formation in rice(Oryza sativa L.)[J].Journal of Experimental Botany,203,54:2701-2708.
[6] Bhumica Singla,Archana Chugh,Jitendra P,et al.An early auxin-responsive Aux/IAA gene from wheat(Triticum aestivum)is induced by epibrassinolide and differentially regulated by light and calcium[J].Journal of Experimental Botany,2006,57(15):4059-4070.
[7] Baluska F,Barlow P W,Volkmann D.Complete disintegration of the microtubular cytoskeleton precedes its auxin-mediated reconstruction in postmitotic maize root cells[J].Plant Cell Physiol,1996,37:1013-1021.
[8] Xianting Wu,Paula McSteen.The role of auxin transport during inflorescence development in maize(Zea mays,Poaceae)[J].American Journal of Botany,2007,94(11):1745-1755.
[9] Hironori Fujita,Kunihiko Syono.Genetic analysis of the effects of polar auxin transport inhibitors on root growth in Arabidopsis thaliana[J].Plant Cell Physiol,1996,37:1094-1101.
[10] Ken Haga,Moritoshi Iino.Asymmetric distribution of auxin correlates with gravitropism and phototropism but not with autostraightening(autotropism)in pea epicotyls[J].Journal of Experimental Botany,2006,57(4):837-847.
[11] Naoki Sakurai,Yoshio Masuda.Auxin-indnced changes in barley coleoptile cell wall composition[J].Plant Cell Physiol,1978,19:1217-1223.
[12] Maaike de Jong,Celestina Mariani,Wim H.Vriezen.The role of auxin and gibberellin in tomato fruit set[J].J Exp Bot,2009,60:1523-1532.
[13] 杨泽峰,顾世梁,许花,等.拟南芥和水稻cystatin 基因家族的生物信息学分析[J].扬州大学学报:农业与生命科学版,2007,28(3):51-57.
[14] Sonnhammer E L,Eoldy S R,Durbin R.Pfam:A comp rehensive database of protein famdies based on seedalignmenbs[J].Proteins,1997,28(3):405-420.
[15] Quevillon E,Silventinen V,Pillai S,et al.Interproscan:Protein domains identifies[J].Nucleic Acids Res,2005,33:116-120.
[16] 陈磊,李学伟,朱砺,等.猪CystatinB基因cDNA 克隆及遗传多态性分析[J].中国农业科学,2008,41(7):2120-2127.
[17] Andrew W,Woodward,Bartel B.Auxin:regulation,action and interaction[J].Annals of Botany,2005,95:707-735.
[18] Tian C,Muto H,Higuchi K,et al.Disruption and overexpression of auxin response factor 8 gene of Arabidopsis affect hypocotyl elongation and root growth habit,indicating its possible involvement in auxin homeostasis in light condition[J].The Plant Journal,2004.40(3).
[19] 牛西午,詹海仙,畅志坚,等.不同激素浓度对柠条茎段组织培养及植株再生的影响[J].华北农学报,2005,20(1):35-37.
[20] http://baike.baidu.com/view/1714201.htm.
[21] 苏中滨,战守义,郑萍,等.作物高光效株型数字化设计方法研究[J].农业工程学报,2008,24(1):203-207.
[22] 王冰,李家洋,王永红.生长素调控植物株型形成的研究进展[J].植物学通报,2006,23(5):443-458.
[23] Ellis C M,Nagpal P,Young J C,et al.Auxin response factor1 and auxin response factor2 regulate senescence and floral organ abscission in Arabidopsis thaliana[J].Development,2005,132:4563-4574.
