nsdA (Negative regulator of Streptomyces differentiation) is globally negative regulator gene first found in Streptomyces coelicolor.In this study,nsdA gene of Streptomyces roseoflavus strains Men-myco-93-63 was amplified using the primer sequences designed from.Streptomyces coelicolor A3(2).After sequencing,Blastn,and Blastx,it showed that the cloned fragment from Streptomyces roseoflavus Men-myco-93-63 with the primer of nsdA-2R and nsdA-2L compairing with reported nsdA conservative nucleotide sequence homology on the region reached 99%.The fulllength gene sequence of Men-myco-93-63 nsdA gene amplification with primers of nsdA WZ-R and nsdA WZ-L had a complete open reading frame encoding 369 amino acids,with the Streptomyces lividans and Streptomyces coelicolor A3(2) nsdA gene at nucleotide sequence 100%,and amino acid sequence homology up to 99%.The fulllength nsdA gene of Streptomyces roseoflavus Men-myco-93-63 was named nsdAmgh,which will promote the obtaintion of high-yielding antibiotic strains of Streptomyces roseoflavus Men-myco-93-63 through interrupting the negative regulator.
SHEN Feng-ying
,
LIU Li-qiang
,
WU Wei-gang
,
LI Ya-ning
,
LIU Da-qun
. Cloning and Sequence Analysis of nsdAmgh Gene in Streptomyces roseoflavus Men-myco-93-63[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(5)
: 77
-80
.
DOI: 10.7668/hbnxb.2009.05.017
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