The purpose of this study was to improve the lysine content in corn，Sb401 gene was introdued into imma- ture embryo callus using particle bombardment．Regeneration into plants by selection for 1．5，3．0 mol/L bialaphos resist- ance. PCR detection in the obtained by plants and protein dipsticks testing．The results show that 38 transgenic plants were identified by PCR from 58 regenerated transformed plants obtained by selection for bialaphos resistance，31 transgen- ic plants was identified by test trip．It turned out that 31 transgenic plants has introduced target gene．
TIE Shuang-gui
,
CHEN Xiao-jie
,
YUE Run-qing
,
QI Jian-shuang
,
YAN Shu-feng
,
HAN Xiao-hua
,
XU Yu-ge
,
LIN Hong
. Acquirement of the Transgenic Maize Harboring Sb401 Gene[J]. Acta Agriculturae Boreali-Sinica, 2013
, 28(4)
: 66
-68
.
DOI: 10.3969/j.issn.1000-7091.2013.04.012
[1] 张丽颖，王金君，刘志新，等． 高赖氨酸玉米的发展前 景[J]. 杂粮作物，2004，24(6): 361-362．
[2] Mertz E T，Bates L S，Nelson O E，et al． Mutant gene changes protein composition and increase lysine content of endosperm[J]. Science，1964，145: 279-280．
[3] 张秀君，刘俊起，赵 倩，等． 用基因枪将高赖氨酸蛋 白基因导入玉米及转基因植株的检测[J]. 农业生物 技术学报，1999，7(4): 363-367．
[4] 贾炜珑，杨丽莉，周小梅，等． 基因枪转化玉米愈伤组 织参数的优化[J]. 华北农学报，2001，16(4): 77-80．
[5] 蒋武生，郅玉宝，耿建峰，等． 玉米自交系幼胚培养与 基因枪转化研究[J]. 河南农业科学，2001(10): 4-5．
[6] Liu J，Seul U，Thompson R，et al． Cloning and character-ization of a pollen-specific cDNA encoding a glutamic-acid-rich protein (GARP) from potato Solanum berthault-ii[J]. Plant Molecular Biology，1997，33: 291-300．
[7] 岳同卿． 转 BtCry1Ah 基因抗虫玉米的研究[D]. 北京: 中国农业科学院，2009．
[8] Vain P，McMullen J J． Osmotic treatment enhances parti-cle bombardment-mediated transient and stable transfor-mation of maize[J]. Plant Cell Reports，1993．
[9] 彭存智，刘志昕，郑学勤． 植物转基因沉默研究进展 对策及应用[J]. 生命的化学，2001，21(5): 407-410．
[10] 铁双贵，柏 松，岳润清，等． 基因枪介导法获得转 BtCry1Ac 基因抗虫玉米植株的研究[J]. 玉米科学，2012，20(5): 54-58．
