To study the regulation of upstream regulatory region polymorphisms on BoLA- DRB3 gene expres- sion，PCR- SSCP and DNA sequencing were used to identify the linkage relationship between BoLA- DRB3* exon2 and BoLA- DRB3 URR．Combined with the predicted results of binding mode between different URR and transcrip- tion factor，three linkage types were selected to detect the BoLA-DRB3 mRNA levels by relative Real- time PCR ex- periments． The results showed that the expression abundances of BoLA-DRB3 varied in different linkage types.The expression level of mRNA of BoLA- DRB3 gene linked with H17H17 was significant higher than H1H1 and H5H6 (P＜0.01)．This preliminary proved that the polymorphism of upstream regulatory region can cause the expression change of BoLA- DRB3 structural gene．
WANG Xue-qing
,
LI Kui-ying
,
PEI Cui-juan
,
ZHANG Feng
,
WU Zhan-jun
,
LIU Xiao-hu
,
MA Shu-lin
,
ZHANG Xin-tong
,
WANG Kun
. Expression of BoLA- DRB3 mRNA in Chinese Holstein Cows[J]. Acta Agriculturae Boreali-Sinica, 2013
, 28(4)
: 19
-22
.
DOI: 10.3969/j.issn.1000-7091.2013.04.004
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