The complete gene encoding the structural protein of FMDV (P1) was subcloned into expression vector pET228α(+),resulting in the fusion expression plasmid pET2P1.After transformed into E.coli (DE3) and induced by IPTG,the results of SDS2PAGE showed that the fusion protein was expressed in high level.The molecular weight of the fusion protein was 85 kDa and the expressed products were significant at inclusion body.Western blotting was performed to confirm that the expressed fusion protein could specifically react with antiserum against FMDV.The fusion protein were further purified and vaccined antigen based on the purified protein was developed.
CHANG Hui-yun
,
CONG Guo-zheng
,
DU Jun-zheng
,
SHAO Jun-jun
,
LIN Tong
,
FENG Jin-rui
,
LIU Ping
. Expression of the Capsid :Precursor Polypeptide P1 of Foot2and2mouth Disease Virus Type C and Analysis of Its Biology Activity[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(5)
: 7
-10
.
DOI: 10.7668/hbnxb.2009.05.002
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