In this study， two markers Vp1A3 and Vp1B3 associated with PHS tolerance were used to evaluate the 46 white-grained wheats， in order to select out the cultivar which had the strong capacity of resistance PHS tolerance，and amplify these resistance PHS cultivars in wheat production and breeding for resistance PHS． In this study，total thirteen Vp-1A haplotypes，which were Vp-1Aam，Vp-1Aan，Vp-1Aao，Vp-1Abm，Vp-1Abn，Vp-1Agm，Vp-1Agn，Vp-1Ahm，Vp-1Aho，Vp-1Aim，Vp-1Ain，Vp-1Aio and Vp-1Ajm with frequencies of 17.4%，8.7%，2.2%，4.3%，4.3%，6.5%，2.2%，2.2%，2.2%， 15.2%， 28.3%，4.3% and 2.2% respectively，were detected by STS marker Vp1A3，and three Vp-1B haplotypes，which were Vp-1Bc，Vp-1Ba and Vp-1Bb with frequencies of 71.7%，17.4%and 4.3%， respectively，were detected by STS marker Vp1B3． In addition， there were five cultivars were selected out，which were Mianyang 31，Yumai 35，Fengmai 24，CA0489 and Shandong 928802，having the gene about resistance PHS． The haplotypes of Vp-1 in these five cultivars，Mianyang 31，Yumai 35，Fengmai 24，CA0489 and Shandong 928802，were Vp-1Agm and Vp-1Bc，Vp-1Agm and Vp-1Bc，Vp-1Agm and Vp-1Bc，Vp-1Aam and Vp-1Bb，Vp-1Abm and Vp-1Bb， respectively． Therefore， selecting efficiency for seeds dormancy could be improved by using a combination of the STS markers Vp1A3 and Vp1B3．
Yang Yan
,
Wang Xiaoli1
,
Liu Shixin1
,
Zhao Xianlin
. Determination of Resistance Genotypes with STS Markers in Current Wheat Cultivars[J]. Acta Agriculturae Boreali-Sinica, 2013
, 28(3)
: 183
-188
.
DOI: 10.3969/j.issn.1000-7091.2013.03.033
[1] Biddulph T B，Plummer J A，Setter T L， et al． Influence of high temperature and terminal moisture stress on dormancy in wheat ( Triticum aestivum L．) [J]． Field Crops Res， 2007， 103: 139 - 153．
[2] Rajender S． Identification and validation of genomic regions associated with pre-harvest sprouting resistance in white-grained wheat ( Triticum aestivum L．) [D]． Saskatoon， Saskatchewan: University of Saskatchewan， 2008．
[3] Clarke F R，Clarke J M，DePauw R M， et al． Strategic approach to mitigating weather induced defects of wheat quality [J]． Euphytica， 2005， 143: 285 - 290．
[4] Xiao S H，Zhang X Y，Yan C S，et al． Germplasm improvement for preharvest sprouting resistance in Chinese white-grained wheat: Anoverview of the current strategy [J]． Euphytica， 2002， 126: 35 - 38．
[5] 中国天气网． 湖北襄樊阴雨阻小麦收割多数发芽霉变损失严重[EB/OL]． 2009 - 06 - 01． http: / /www． weather．com． cn /static /html /article /20090601 /33844． shtml．
[6] 罗家传，韦胜利． 河南省主栽小麦品种( 系) 的抗穗发芽试验[J]． 河南农业科学， 2001( 2) : 11 - 12．
[7] 薛香，王巧玲． 小麦穗发芽抑制剂的筛选[J]． 河南农业科学， 2005( 9) : 22- 24．
[8] Lohwasser U，Rder M S，Bǒ rner A． QTL mapping of the domestication traits pre-harvest sprouting and dormancy in wheat ( Triticum aestivum L．) [J]． Euphytica， 2005， 143:247 - 249．
[9] Atsushi T，Yoichi A． Effect of seed coat color on seed dormancy in different environments [J]． Euphytica，2002，126: 99 - 105．
[10] Zhang X Q，Li C D，Tay A，et al． A new PCR-based marker on chromosome 4AL for resistance to pre-harvest sprouting in wheat ( Triticum aestivum L．) [J]． Mol Breeding， 2008， 22( 2) : 227 - 236．
[11] Liu S B，Cai S B，Robert G， et al． Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco [J]． Theor Appl Genet，2008，117: 691 - 699．
[12] Mares D，Mrva K，Cheong J，et al． A QTL located on chromosome 4A associated with dormancy in white-and red-grained wheats of diverse origin [J]． Theor Appl Genet， 2005， 111: 1357 - 1364．
[13] McCarty D R，Hattori T，Carson C B， et al． The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator [J]． Cell， 1991， 66: 895 - 905．
[14] McKibbin R S，Wilkinson M D，Bailey P C， et al． Transcripts of Vp-1 homologues are mis-spliced in modern wheat and ancestral species [J]． Proc Natl Acad Sci USA， 2002， 99: 10203 - 10208．
[15] 刘世鑫． 休眠基因Viviparous-1A 在中国小麦3A 染色体上等位变异的鉴定及STS 分子标记的开发[D]． 呼和浩特: 内蒙古农业大学， 2012．
[16] Yang Y，Zhao X L，Xia L Q， et al． Development and validation of a Viviparous-1 STS marker for pre-harvest sprouting resistence in Chinese wheat [J]． Theor Appl Genet， 2007， 115: 971 - 980．
[17] Gale K R，Ma W，Zhang W， et al． Simple high-throughput DNA markers for genotyping in wheat[C]/ /Eastwood R． 10th Australian wheat breeding assembly proceedings， 2001: 26 - 31．
[18] Bassam B J，Caetano-Anolles G，Gresshoff P M． Fast and sensitive sliver staining of DNA in polyacrylamide gels[J]． Annal Biochem， 1991， 196: 80 - 83．
[19] Sun Y W， Jones H D，Yang Y， et al． Haplotype analysis of Viviparous-1 gene in CIMMYT elite bread wheat germplasm[J]． Euphytica， 2012， 186: 25 - 43．
[20] 孙果忠，游光霞，孙京燕，等． 我国小麦主推品种穗发芽抗性鉴定及相关分子标记的评价[J]． 华北农学报， 2010， 25( 4) : 6 - 11．
[21] Yang Y，Ma Y Z，Xu Z S， et al． Isolation and characterization of Vp-1 genes in wheat varieties with distinct preharvest sprouting tolerance and ABA sensitivity[J]． JExp Bot， 2007， 58( 11) : 2863 - 2871．
[22] 杨燕，张春利，陈新民，等． 应用穗发芽和发芽指数及STS 标记Vp1B3 鉴定小麦抗穗发芽基因型[J]． 麦类作物学报， 2007， 27( 4) : 577 - 582．
