To investigate the effects of cold acclimation on the cold tolerance, the leaves of chrysanthemum ( Chrysanthemum morifolium)were treated at 4 ℃for 5 hours,and then the changes of electrolytic leakage,content of mal2 ondialdehyde level (MDA),soluble protein,proline and activity of 5′2 nucleotidase,superoxide dismutase (SOD) and per2 oxidase (POD)were measured after low temperature stress with different times (0. 5 - 5 h). The results showed that the electrolytic leakage and the content of MDA in leaves treated with cold acclimation were lower than those of non2treated, the contents of proline and the activities of SOD and 5′2 nucleotidase kept higher levels compared with those of controls. The results indicated that the cold tolerance of chrysanthemum was improved by cold acclimation treatment.
LI Yun
,
YANG Ji-shuang
,
ZHANG Gang
,
CHEN Duan-fen
. Effects of Cold Acclimation on Physiological Metabolism of Chrysanthemum under Low Temperature Stress[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(4)
: 179
-182
.
DOI: 10.7668/hbnxb.2009.04.035
[1] 郑路,傅玉兰,陈树桃,等.菊花抗寒性与营养特性的研究[J].园艺学报994,21 (2):185-188.
[2] Anderson N,Gesick E.Phenotypic markers for selection of winterhardy garden chrysanthemum (Dendranthema×grandiflora Tzvelv.)genotypes[J].Sci Hort,200401:153-167.
[3] 许瑛,陈发棣.8个小菊品种的低温半致死温度及其抗寒适应性[J].园艺学报,2008,35(4):559-564.
[4] 李明玉,曹辰兴,于喜艳.低温锻炼对冷胁迫下黄瓜幼苗保护性酶的影响[J].西北农业学报,20065(1):160-164.
[5] 王瑞云,任有蛇,岳文斌,等.低温胁迫对苜蓿幼苗存活及生理生化指标的影响[J].激光生物学报,20065(4):342-348.
[6] Ryypp(o) A,Repo T,Vapaavuori E.Development of freezing tolerance in roots and shoots of Scots pine seedlings at nonfreezing temperatures[J].Can J Forest Res998,28:557-565.
[7] 张宪政.植物生理学实验技术[M].沈阳:辽宁科学技术出版社989:310-312.
[8] 李柏林,梅慧生.燕麦叶片衰老和活性氧代谢的关系[J].植物生理学报9895 (1):6-12.
[9] 李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000.
[10] 薛应龙.植物生理学实验手册[M].上海:上海科学技术出版社985:67-70.
[11] 潘杰,孙龙华,简令成.不同抗冷性水稻质膜5'-核苷酸酶活性的生化及细胞化学研究[J].科学通报992,37(7):653-656.
[12] 李美如,刘鸿先,王以柔.低温对水稻幼苗叶片质膜5'-核苷酸酶活性的影响[J].植物生理学通讯996,32(3):195-197.
[13] 刘鸿先,曾邵西,王以柔,等.低温对不同耐寒力黄瓜幼苗子叶各细胞器官中SOD的影响[J].植物生理学报9851(1):48-57.
[14] Kratsch H A,Wise R R.The ultrastructure of chilling stress[J].Plant Cell Environ,2000,23:337-350.
[15] 佘文琴.白兰花的耐寒性及其越冬期保护酶活性的变化[J].福建农业大学学报,2000(29):440-443.
[16] 钱芝龙,丁犁平,曹寿椿.低温胁迫对辣椒幼苗膜脂过氧化水平及保护酶活性的影响[J].园艺学报994,21(2):203-204.
[17] Yang S A,Endo K.Acclimation temperature affects activities of 5'-nucleotidase and acid phosphatase and lipid and fatty acid composition in carp muscle microsomes[J].J Food Sci994,59 (5):1009-1012.
[18] Eastwell K C,Stumpf P K.The presence of 5'-nucleotidase in Swiss chard chloroplasts Beta vulgaris,comparison of nucleotide metabolism in Swiss chard and spinach chloroplasts[J].Biochem Biophys Res Comm98208(4):1690-1694.
