The DNA of P1 gene of Foo-t and-mouth disease virus Asia1/HeB was amplified and cloned, and then sequenced. Amino acid sequence of P1 on secondary structure, flexibility, hydrophilicity, accessibility and ant igenicity were analyzed using DNAStar Protean software,with the B cell epitopes to be predicted.The results showed that the P1 region of FMDV Asia1/HeB is 2 199 bp in length and codes for a polypeptide of 733 amino acids, including complete open reading frame. The structural protein of VP1,VP2,VP3,VP4 gene consists of 633, 654, 657, 255 bp and codes for 211, 218, 219, 85 amino acids respect ively. The secondary structure of P1 region was relatively complicated consisting of more B sheet regions and turn regions.There were predominant B cell epitopes in VP1,VP2, VP3 and a small amounts in VP4. Comparing with the epitopes that have been published, the predict results have a high reliability. This study would be helpful for identification of B cell epitopes for structural protein of FMDV Asia1/HeB strain using experimental methods and research of reverse vaccine of FMDV.
ZHANG Zhong-wang
,
ZHANG Yong-guang
,
WANG Yong-lu
,
PAN Li
,
FANG Yu-zhen
,
LIU Li-kuan
,
JIANG Shou-tian
,
LU Jian-liang
,
ZHOU Peng
,
ZHANG Yu
,
ZHANG Shu-gang
,
DU Jin-xin
,
LI Zheng-feng
. Cloning,Sequencing and B Cell Epitopes Prediction of Structural Protein Gene of a Foot-and-Mouth Disease Virus Strain Asia1/HeB[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(4)
: 57
-63
.
DOI: 10.7668/hbnxb.2009.04.012
[1] Pereira H G.Foot-and-mouth Disease[M]//Gibbs E P J.Virus Diseases of Foot Animals.London:Academic Press Inc981:333-363.
[2] Eric B,Carmen M,Ruiz-Jarabo,et al.Foot-and-mouth Disease Virus Lacking the VP1 G-H loop:The Mutant Spectrum Uncovers Interactions among Antigenic Site for Fitness Gain[J].Virology,2001,288:192-202.
[3] Hoop J P,Wood K R.Prediction of protein antigenic determinants from amino acid sequences[J].Immunology981,78(6):3824.
[4] Kyte J,Doolittle R F.A Simple method for displaying hydropathic character of a protein[J].J Mol Biol983,57(1):105.
[5] Tsche H.Modern methods in protein and nucleic acid research[M].1th ed.New York:Walter de Gruger Berlin990,231.
[6] Welling G W,Weijer W J,Vander Z R,et al.Prediction of sequential antigenic regions in proteins[J].FEBS Lett98588(2):215.
[7] Karplus P A,Schultz G E.Prediction of chain flexibility in proteins[J].Immunology985,72(2):212.
[8] Gershoni J M,Stem B,Venisova G.Combinatorial libraries,epitope structure and the prediction of conformations[J].Immunol Today9978(5):108.
[9] 来鲁华.蛋白质的结构预测与分子设计[M].第1版.北京:北京大学出版社993:49.
[10] 吴玉章,朱锡华.一种病毒蛋白B细胞表位预测方法的建立[J].科学通报994,39(24):2275-2279.
[11] Doel T R.FMD vaccines[J].Virus Res,2003,91(1):81-99.
[12] Rob H M,Langeveld P M,Wim M M,et al.Synthetic peptide viccines:unexpected fullfillment of discarded Hope[J].Biologicals,2001,29(3-4):233-236.
[13] 吴玉章,刘茂昌,贾正才,等.HBV新型免疫原的设计、合成及免疫原性研究[J].第三军医大学学报,2000,22(10):919-923.
[14] Kolaskar A S,Kulkami Kale U.Prediction of three dimensional structure and mapping of conformational epitopes of envelope glycoprotein of Japanese encephalitis virus[J].Virology999,261(1):31-42.
