BAC subclone library is an important platform for sequencing the whole genome or some specific regions. In order to accelerate the process of sequencing the wheat genome, a BAC clone (BAC790010) fromLangdon librarywas selected as material, and then was broken by DNA shearing instrument to produce many DNA fragments with different sizes, fromwhich the 3- 5 kb DNA fragments were retrieved and cloned to TOPO vector and then the subclone librarywas constructed that was suitable for shotgun sequencing. This BAC subclone librarywould provide a platformfor gene cloning and genome research of wheat.
GAO Shuang-cheng
,
WANG Shi-hua
,
SHI Jiang
,
KONG Xiang-sheng
,
SHI Guo-an
. Construction of Subclone Library for Wheat Megabase BAC DNA[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(4)
: 23
-25
.
DOI: 10.7668/hbnxb.2009.04.005
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