Extraction of High Quality RNA from Whangkeumbae Pear

  • SUN Wen ,
  • SHI Hai-yan ,
  • ZHANG Yu-xing
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  • Horticultural College, Hebei Agricultural University, Baoding 071001, China

Received date: 2013-01-25

  Online published: 2014-10-14

Abstract

Isolation of high-quality RNA is the necessary premise ofgene cloning and Northern blot. The Whangkeumbae Pear has the characteristic of the high level of polyphenol,polysaccharides and other secondary me-tabolities. Numerous methods of the predecessors have been taken into consideration,and three of them were chosen to be utilised to compare. The result shows that:modified 3% CTAB method and TIANGEN RNAplant plus Reagent were suitable for total RNA extraction from Whangkeumbae Pear,but TIANGEN RNAplant plus Reagent is more suitable. The electrophoresis of RNA was showed less degradation and higher purity.

Cite this article

SUN Wen , SHI Hai-yan , ZHANG Yu-xing . Extraction of High Quality RNA from Whangkeumbae Pear[J]. Acta Agriculturae Boreali-Sinica, 2013 , 28(2) : 70 -72 . DOI: 10.3969/j.issn.1000-7091.2013.02.013

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