Aborted,fatal death and died after postnatal′ s pigletswere collected in Shandong province. The PCR,RT- PCR,ELISA tests and cell culture were performed to detect Porcine Reproductive Disturbance:classical swine fever virus ( CSFV), pseudorabies virus( PRV), porcine circovirus ( PCV), porcine parvovirus ( PPV)and porcine reproductive and respiratory syndrome virus ( PRRSV). From the detection results,CSFV,PCV-2 were positive,PRRSV, PRV, PPV were negative. The result indicated that the swine field were infected by CSFV and PCV-2,but CSFV was severity.
WU Su li,, SUN Hui ling, ZHANG Ping, ZHANG Pei jun, QIAN Yong hua, GAo Li na
. Diagnosis Research of Porcine Reproductive Disturbance in Shandong Province[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(3)
: 92
-95
.
DOI: 10.7668/hbnxb.2009.03.020
[1] 殷震,刘景华主.动物病毒学[M].北京:科学出版社,1997:631-664.
[2] 蔡宝祥.家畜传染病学[M].第4版.北京:中国农业出版社,2001:201-213.
[3] 圈华,贺小龙.母猪繁殖障碍疾病及原因(下)[J].畜牧兽医,2006(8):53-54.
[4] 吴志明,闫若潜,赵明军,等.采用PCR方法对猪场猪瘟病毒、猪繁殖障碍与呼吸道综合征病毒、猪圆环病毒2型混合感染的诊断研究[J].中国畜牧兽医,2007,34(1):86-89.
[5] 王琴,宁宜宝.猪瘟免疫失败主要原因的解析[J].中国兽医杂志,2005,41(6):61-63.
[6] 唐丽,赵玉军,许立博.猪圆环病毒2型的研究进展[J].中国畜牧兽医,2008,35(4):10-106.
[7] 李琼,张以芳,王军.一起猪瘟与猪繁殖障碍与呼吸道综合征混合感染的RT-PCR检测方法[J].中国畜牧兽医,2007,34(12):95-96.
[8] Molitor T W,Oraveerakul K,Zhang Q,et al.Polymerase ehainreacfion(PCR) amplification for the detection of porcine pfltrvo-virus[J].Viral Methods,1991,32(2):201-211.
[9] 李维华,任慧英,温建新,等.PCV2、PPV、PRV、PRRSV和CSFV复合PCR检测方法的建立[J].中国预防兽医学报,2008,30(5):379-383.
[10] 宁宜宝,赵耕,王琴.3种非猪瘟病毒单独或混合感染对猪瘟弱毒疫苗免疫效力的影响[J].中国兽医学报,2004,24(2):112-114.
[11] 赵耘,秦玉明,张广川,等.猪瘟病毒、猪细小病毒、猪繁殖与呼吸综合征病毒、猪伪狂犬病毒多重PCR方法的建立以及初步应用[J].中国兽医杂志,2007,43(2):3-6.
