Five invertases were investigated by semi2quantitative RT2PCR in different developing stages of the fruit, using genotype tomatoes,″ Momotaro″. The invertase genes expressions of tomato seedling under sub2high temperature,sub2 low temperature,NaCl salinity stress and osmotic stress were further studied. The results suggested that the expanding pe2 riod of fruit is affected by the expression of Lin5,Lin6 and Lin8.Only Lin8 expressed in mature green stage,while Lin6, Lin8 and AI2 expressed distinctly. The results showed that different members of acid invertase gene family regulated the different periods and tissues of tomato fruits development. Suboptimal temperature had significant influence on AI2. Whether sub2low temperature or sub2high temperature,both of them inhibited gene expression significantly,however,they have little effect on the expression of the cell wall2binding acid invertase genes (Lin5,Lin6 and Lin7) and have inhibited effect on Lin8. Lin5 and Lin7 have no expression in osmotic stress and salinity stress;however,the expressions of Lin6 and Lin8 are inhibited by osmotic stress and salinity stress. The expression was low when treated for three days,and for five days it was totally inhibited. AI2 is sensitive to osmotic stress whose expression is totally inhibited when treated for three days,meanwhile under prolonging the time of the salt stress,the expression is low,then completely disappeared.
SUN Wei
,
JIANG Jing
,
LI Tian lai
,
LU Shao wei
. Expression Analysis of Acid Invertase Gene Family in the Development of Tomato Fruits and under Adversity Stress[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(3)
: 37
-40
.
DOI: 10.7668/hbnxb.2009.03.008
[1] Tang G Q,Luscher M,Strm A.Antisense repression of vacuo-lar and cell wall invertase in transgenic carrot alters early plant development and sucrose partitioning[J].The Plant cell,1999,11(1):177-189.
[2] Sturm A,Tang G Q.The sucrose cleaving enzymes of plants are crucial for development,growth and carbon partitioning[J].Trends in Plant Science,1999,4(10):401-407.
[3] Klann E M,Chetelat R T,Bennett A B.Expression of acid in-vertase gene controls sugar composition in tomato(Lycopersi-con)fruit[J].Plant Physiology,1993,103(3):863-870.
[4] Dickison C D,Altabella T,Chrispeels M J.Slow-growth phe-notype of transgenic tomato expressing apoplasfic invertase[J].Plant Physiol,1991,95:420-425.
[5] Yelle S,Chetelat R T,Dorais M.Sink metabolism in tomato fruit Ⅳ.Genetic and biochemical analysis of sucrose accumu-lation[J].Plant Physiol,1991,95:1026-1035.
[6] Eyal F,Dani Z.Functional divergence or a syntenic invertase gene family in tomato,potato and Arabidopsis[J].Plant Phys-iology,2003,(131):603-609.
[7] 王永章,张大鹏.果糖和葡萄糖参与诱导苹果果实酸性转化酶翻译后的抑制性调节[J].中国科学,2002(32):
[8] 张洁,齐明芳,李天来.亚高温处理对番茄叶片光合作用及糖类的影响[J].沈阳农业大学学报,2006,37(3):304-307.
[9] 黄芳.夜间温度对番茄生长的影响[D].泰安:山东农业大学,2002.
[10] 程智慧,孟焕文,Julie D.水分胁迫对番茄幼苗转化酶表达及糖代谢的影响[J].园艺学报,2002,29(3):278-279.
[11] 程智慧,高芸,孟焕文.盐胁迫对番茄幼苗转化酶表达及糖代谢的影响[J].西北农林科技大学学报,2007,1(35):184-188.
[12] 姜晶,李天来.番茄蔗糖代谢关键基因的分子生物-学研究[J].生物技术,2005,15(1):82-85.
[13] 姜晶,李伟,李天来.乙酰水杨酸对盐胁迫下番茄蔗糖代谢关键酶活性与基因表达的影响[J].沈阳农业大学学报,2007,38(1):30-34.