It was found that glyceralehyde232phosphate dehydrogenase (GAPDH)was frequently uP2rgulated expres2 sion in salt and drought stress treatment experiments of foxtail millet seedlings in our previous work and from literature. For detail annotation of the function of G APDH in tolerance reaction of abiotic stress,full length cDNA of foxtail millet G APDH gene was cloned using RACE technology in this paper. This full length cDNA contains a 1008 bp open2reading2 frame which encoding a 338 amino acid peptide with an estimated molecular weight of 35 911. 03 Da. Sequence compari2 son and phylogenetic analysis indicated that the foxtail millet G APDH cDNA was highly identical with its ortholog from maize and the next most similar orthologs come from two other cereals,rice and wheat. The possible functions and related topics were discussed.
CUI Runli
,
WANG Yong fang
,
ZHI Hui
,
LI Wei
,
LI Haiquan
,
HUANG Zhan jing
,
DIAo Xian min
. Cloning and Structure Analysis of Foxtail Millet APDH Gene[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(3)
: 10
-14
.
DOI: 10.7668/hbnxb.2009.03.003
[1] 聂新辉,曲延英,尤春源,等.mRNA差异显示分离棉花抗旱耐盐基因的相关eDNA片段[J].新疆农业大学学报,2007,30(4):72-75.
[2] Jinpeng Zhang,Tingsong Liu,Junjie Fu,et al.Construction and application of EST library from Setaria italica in reaponse to dehydration stress[J].Genomics,2007(90):121-131.
[3] 鲁国东,郑学勤.稻瘟病菌3-磷酸甘油醛脱氢酶基因的克隆及序列分析[J].热带作物学报,2005,4:83-89.
[4] Forhman M A,Dush M K,Martin G R.Rapid production of full-length cDNAs from rare transcripts:amplication using a single gene-special oligonucleotide primer[J].Proe Nail A-cad sci USA,1985,85:8998-9002.
[5] 智慧,刁现民,吕芃,等.人工盐胁迫法鉴定谷子及狗尾草物种耐盐基因型[J].河北农业科学,2004,8(4):9-15.
[6] Wang Zining,Zhang Jinsong.Cloning and characterization of the Na+/H+antiport genes form triticum aestivum[J].Acta Botaniea Siniea,2002,44(10):1203~1208.
[7] 崔润丽,智慧,王永芳,等.谷子DnaJ蛋白基因的克隆[J].华北农学报,2007,8:9-13.
[8] 王镜岩,朱圣庚,徐长法.生物化学[M].北京:高等教育出版社,2006:607-610.
[9] 李富生,何丽莲.植物对非生物胁迫的生理响应及甘蔗抗旱抗寒性研究进展[J].亚热带农业研究,2004,11(1):15-20.
[10] 程建峰,潘晓云,刘宜柏,等,快速鉴定稻种资源抗旱性的生理指标筛选及其遗传背景[J].西北农业学报,2005,5:529-533.
[11] 罗丽娟.植物分类学[M].北京:中国农业大学出版社,2007:198-200.
[12] 吴龙妍,张建,田志刚,等.基于生物组学的免疫功能基因的发现[J].中国药学杂志,2006,21:92-96.