To obtain moRe wheat( TRiticum aestivum L． ) stRess-Response genes，a phospholipase D gene，named TaPLDα，was cloned by RT-PCR． And the pRotein of this gene was also pRedicted and studied thRough the bioinfoRmatics analysis method． The Results showed that the laRgest open Reading fRame of TaPLDα gene has 2 394 bp in length and encoded a polypeptide of 812 amino acid Residues． The estimated moleculaR weight and isoelectRic point of the putative pRotein weRe 92 kDa and 5． 30， Respectively． Sequence analysis showed that the amino acid sequence encoded by TaPLDα gene contained N-teRminal C2 domain and two HKD motifs． TaPLDα pRotein was a stable hydRophilic pRotein and located in the cytoplasm． The pRedicted secondaRy stRuctuRe composition foR the pRotein has about 28． 82% alpha helixes，20． 07% extended stRand，6 ． 03% beta tuRn and 45． 07% Random coil． The TaPLDα had no signal peptide and tRansmembRane helices． CompaRed with Rice，maize and ARabidopsis，amino acid sequence encoded by TaPLDα was almost conseRved． The phylogenic tRee showed that TaPLDα was most similaR to PLDα pRotein fRom daRnel， Rice and maize．
WANG Jun-bin
,
LI Ming
,
DING Bo
,
BAO Shu-guang
,
WANG Rui
,
XIE Xiao-dong
. Cloning and Sequence BioinfoRmatics Analysis of Phospholipase Dα Gene fRom Wheat[J]. Acta Agriculturae Boreali-Sinica, 2013
, 28(1)
: 117
-122
.
DOI: 10.3969/j.issn.1000-7091.2013.01.022
[1] Hanahan D J，Chaikoff I L． A new phosphoRus-containing lipids of the caRRot[J]． J Biol Chem，1947，168: 233 - 240．
[2] Wang X，Xu L，Zheng L． Cloning and expRession of phosphatidylcholine- hydRolyzing phospholipase D fRom Ricinus communis L[J]． J Biol Chem， 1994， 269: 20312 - 20317．
[3] Wang X． The Role of phospholipase D in signal tRansduction cascade [J]． Plant Physiol， 1999， 121: 456 - 462．
[4] Li M，Hong Y，Wang X． Phospholipase D- and phosphatidic acid-mediated signaling in plants[J]． Biochim Biophys Acta， 2009， 1791: 927 - 935．
[5] Qin C，Wang X． The ARabidopsis phospholipase D family: ChaRacteRization of a calcium independent and phosphatidylcholine- selective PLDζ1 with distinct RegulatoRy domains
[J]． Plant Physiol， 2002， 128: 1057 - 1068．6] Wang C，Wang X． A novel phospholipase D of ARabidopsis that is activated by oleic acid and associated with the plasma membRane[J]． Plant Physiol，2001，127: 1102 - 1112．
[7] Wang X． RegulatoRy functions of phospholipase D and phosphatidic acid in plant gRowth，development，and stRess Responses[J]． Plant Physiol，2005，139: 566 - 573．
[8] Hong Y，Zhang W，Wang X． Phospholipase D and phosphatidic acid signaling in plant Response to dRought and salinity[J]． Plant Cell EnviRon， 2010， 33: 627 - 635．
[9] BaRgmann B O，Laxalt A M， teR Riet B，et al． Multiple PLDs RequiRed foR high salinity and wateR deficit toleRance in plants[J]． Plant Cell Physiol， 2009， 50: 78 - 89．
[10] Hong Y，Pan X，Welti R， et al． Phospholipase Dα3 is involved in the hypeRosmotic Response in ARabidopsis[J]． Plant Cell， 2008， 20: 803 - 816．
[11] Sang Y，Cui D，Wang X． Phospholipase D and phosphatidic acid-mediated geneRation of supeRoxide in ARabidopsis
[J]． Plant Physiol， 2001， 126: 1449 - 1458．
[12] MishRa G，Zhang W，Deng F， et al． A bifuRcating pathway diRects abscisic acid effects on stomatal closuRe and opening in ARabidopsis[J]． Science，2006，312: 264 - 266．
[13] Mane S P，Vasquez-Robinet C，Sioson A A， et al． EaRly PLDα1-mediated events in Response to pRogRessive dRought stRess in ARabidopsis: A tRanscRiptome analysis
[J]． J Exp Bot， 2007， 58: 241 - 252．
[14] 万嗣宝，张斌，战吉宬，等． 桃果实磷脂酶Dα 基因 的电子克隆及序列分析[J]． 中国农业科学， 2009， 42 ( 5) : 1705 - 1712．
[15] Zhang W，Wan X，Hong Y， et al． Plant phospholipase D
[M]/ / Munnik D T ． Lipid signaling in plants ( plant cell monogRaphs) ． BeRlin: SpRingeR publishing company， 2010: 39 - 62．
[16] Ponting C P，PaRkeR P J． Extending the C2 domain family: C2s in PKCs δ，ε，η，θ，phospholipases，GAPs，and peRfoRin[J]． PRotein Sci， 1996( 5) : 162 - 166．
[17] Zheng L，KRishnamooRthi R，Zolkiewski M， et al． Distinct Ca2 + -binding pRopeRties of novel C2 domains of plant phospholipase Dα and β[J]． J Biol Chem，2000，275: 19700 - 19706．
[18] Munnik T，ARisz S A，de VRije T， et al． G pRotein activation stimulates phospholipase D signaling in plants[J]． The Plant Cell， 1995( 7) : 2197 - 2210．
[19] Sung T C，RopeR R L，Zhang Y，et al． Mutagenesis of phospholipase D defines a defines a supeRfamily including a tRans-golgi viRal pRotein RequiRed foR poxviRus pathogenicity[J]． The EMBO JouRnal，1997，16 ( 15) : 4519 - 4530．
[20] Pointing C P，KeRR I D． A novel family of phospholipase D homologues that include phospholipid synthases and putative endonucleases: Identification of duplicated Repeats and potential active site Residues[J]． PRotein Sci， 1996( 5) : 914 - 922．
[21] 时晓伟． CIMMYT 小麦品质育种策略[J]． 天津农业 科学， 2011， 17( 3) : 108 - 111．
[22] 李萌，张爱华，白雪飞，等． 甜瓜玉米黄质环氧化酶 基因的电子克隆及生物信息学分析[J]． 华北农学 报， 2011， 26( S) : 23 - 28．
