In order to investigate the epidemiology of extended-spectrumβ-lactamases-producing E.coli and to detect CTX- M type ESBLs in Henan province,the E.coli strains isolated from chickens and pigs were screened for the presence of ESBLs by phenotype confirmatory test,and theCTX- M type ESBLs were detected by PCR. The results indicated the positive rates of ESBLs among E. coli stains isolated from chickens and pigs were 62. 8 %(59/ 94) and 78. 3 %(36/ 46),respectively.Further PCR amplification results revealed that the positive rate of the CTX- Mtype ESBLs in chickens was 89. 8 %(53/ 59).Of them,there were 14 isolates positive for CTX-M-2 group,26 isolates for CTX- M- 9 group,13 isolates for both CTX-M-2 and CTX-M-9 group.While,the positive rate of the CTX-M type ESBLs in pigs was 69.44 %(25/ 36).Of them,there were seven isolates positive for CTX- M- 2 group,17 isolates for CTX- M- 9 group,one isolate for both CTX-M-2 and CTX-M-9 group.The positive rate of CTX-M type ESBLs-producing Escherichia coli strains is relatively high in Henan province,with the CTX-M-2 group and CTX-M-9 group dominant.
DU Xiang-dang
,
JIAO Xian-qin
,
MO Juan
,
ZHANG Su-mei
,
HOU Chun-bin
,
LI Xin-sheng
. Molecular Detection of CTX-M Type ESBLs Genes among Escherichia coli Strains Isolated from Diseased Chickens and Pigs[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(2)
: 90
-93
.
DOI: 10.7668/hbnxb.2009.02.019
[1] Bradford P A.Extended-spectrum β-Lactamases in the 21st Century:characterization,epidemiology,and detection of this important resistance threat[J].Cin Microbiol Rev,2001,14:933-951.
[2] Jacoby G,Bush K.Amino acid sequences for TEM,SHV and OXA extended-spectrum and inhibitor resistant β-lactamase[EB/OL].http://www,lahey,org/Studies,2007.
[3] Pitout J D,Hossain A,Hanson N D.Phenotypic and molecular detection of CTX-M-laetamases produced by Escherichia culi and klebsiella spp[J].J Clin Microbiol,2004,12:5715-5721.
[4] NCCLs.Performance standards for antimicrobial susceptibility testing; ninth information standard M100-S9[M].Pennsylvania:NCCLs,1999:101-103.
[5] Gniadkowski M,Sehneider l,Palucha A,et al.Cefotaxime-resistant Enterobacteriaceae isolates from a Hospital in Warsaw,Poland:Identification of a new CTX-M-3 cefotaxime-hydro] yzing β-Lactamase that is closely related to the CTX-M-1/MEN-1 enzyme[J].Antimicrob Agents Chemother,1998,42:827-832.
[6] Radice M,Power P,Di Conza J,et al.Early dissemination of CTX-M-derived enzymes in South America[J].Antimicrob Agents Chemother,2002,46:602-604.
[7] Quinteros M,Radiee M,Gardella N,et al.Extended-spectrum β-lactamase in Enterobacteriaceae in Buenos Argentina,public hospitals[J].Antimicrob Agents Chemother,2003,47 (9):2864-2867.
[8] Mushtaq S,Woodford N,Potz N,et al.Detection of CTX-M-15 extended-spectrum β-lactamases in the United Kingdom[J].J Antimicrob Chemother,2003,52 (3):528-529.
[9] 季淑娟,陈亚岗,俞云松,等.浙江省超广谱β-内酰胺大肠埃希菌和肺炎克雷伯菌的基因分型[J].中华传染病杂志,2005,23(2):75-78.
[10] Munday C J,Xiong J,Li C,et al.Dissemination of CTX-M type β-lactamases in Enterobacteriaceae isolates in the People's Republe of China[J].Int J Antimicrobl Agents,2004,23:175-180.
[11] Wang H,Kelkar S,Wu W Y,et al.Clinical isolates of entero bacteriaceae producing extended-spectrum β-lactamases prevalence of CTX-M-3 at a Hospital in China[J].Antimicrob Agents and Chemother,2003,47(2):790-793.
[12] Shen D X,Winoku P,Jones R.Characterization of extended spectrum β-lactamasos Producing Klebsielle pneumoniae from Beijing,China[J].Int J Antimicrob Agents,2001,18:185-188.
[13] Li C R,Li Y,Zhang P A.Dissemination and spread of CTXM extended spectrum β-lactamases among clinical isolates of Klebsielle pneumoniae in central China[J].Int J Antimicrob Agents,2003,22:521-525.
[14] Xiong Z Z,Zhu D M,Wang F,et al.Investigation of extended spectrum β-lactamases in Klebsielle pneumoniae and Escherichia coli from China[J].Diagn Microbiol and Infect Dis,2002,44:195-200.
[15] 张盛斌,刘朝辉.CTX-M型ESBLs的研究进展[J].中国感染与化疗杂志,2006,6(3):212-215.
[16] 高春明,赵守松,李家斌,等.产CTX-M型超广谱β-内酰胺酶大肠埃希菌分布与耐药性分析[J].实用医学杂志,2007,23(11):1745-1747.
[17] 朱戌冬,吴伟元,陈民钧.超广谱β-内酰胺酶CTX-M型新进展[J].中国抗感染化疗杂志,2001,1:109-110.
[18] 李金钟,刘利平.CTX-M型β-内酰胺酶的研究进展[J].国际检验医学杂志,2007,28(6):543-546.
