Granule-bound starch synthase (GBSS),Starch branching enzyme 1 (SBE1) and Starch branching enzyme 3(SBE3) are three major enzymes involved in starch biosynthesis in rice endosperm. The granule-bound starch synthase was encoded by the Wx gene,and the starch branching enzymes were encoded by the Sbe1 and Sbe3 gene.And molecular markers for Sbe1,Sbe3 developed based on sequence diversities between the rice subspecies indica and japonica,together with PCR2AccⅠmarker for Wx gene were used to investigate the genotypes of 183 rice cultivars.The result showed that the developed molecular markers for Wx,Sbe1,Sbe3 could distinguish indica or japonica alleles at three loci.Among all the 183 rice cultivars,eight genotype combinations were observed at Sbe1,Sbe3 and Wx loci. In order to explore the genetic effects of three genes,ANOVA and multiple comparison analysis were conducted. The results showed that rice cultivars with different genotypes exhibited different phenotypes,including amylose content,gel consistency and some of RVA characteristics,and the significant differences were observed. And the genetic effects of indica and japonica alleles at three gene loci were different,Wx gene had significant effects on mainly rice quality traits,followed by Sbe3 and Sbe1. The genetic effects of Sbe1 and Sbe3 in different backgrounds (Wxa, Wxb)were different. Interactions existed among Wx, Sbe1, Sbe3,Interaction was generally detected between Wx and Sbe3.
CHEN Feng
,
SUN Gong-chen
,
YANG Ze-feng
,
ZHANG Shi-yong
,
ZHANG Hong-rui
,
YANG Ya-chun
,
YUAN Shou-jiang
,
ZHANG Zheng-qiu
. The Effects of Starch-synthesizing Genes on Rice Quality[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(2)
: 55
-59
.
DOI: 10.7668/hbnxb.2009.02.012
[1] 李欣,顾铭洪,潘学彪.常见水稻品种稻米品质的研究[J].江苏农学院学报,1987,8(1):1-8.
[2] Sano Y.Differential regulation of waxy gene expression in rice endosperm[J].Theoretical and Applied Genetics,1984,68:467-473.
[3] Hirano H Y,Eiguchi M,Sano Y.A single base change altered the regulation of the Waxy gene at the posttranscriptional level during the domestication of rice[J].Molecular Biology and Evolution,1998,15(8):978-987.
[4] Wang Z Y,Zbeng F Q,Shen G Z,et al.The amylose content in rice endosperm is related to the post-transcriptionul regulation of the Waxy gene[J].The Plant Journal,1995,7(4):613-622.
[5] Wanchana S,Toojinda T,Tragoonrung S,et al.Duplicated coding sequence in the waxy allele of tropical glutinous rice (Oryza sativa L.)[J].Plant Science,2003,165:1193-1199.
[6] 吴洪恺,梁国华,严长杰,等.水稻不同生态型品种间直链淀粉含量的变异及其遗传分析[J].作物学报,2006,32(9):1301-1305.
[7] Han X Z,Hamaker B R.Amylopectin fine structure and rice starch paste breakdown[J].Journal of Cereal Science,2001,34:279-284.
[8] Ong M H,Blanshard J M V.Texture determinants of cooked,parboiled riee.2.Physicochemical properties and leaching behavior of rice[J].Journal of Cereal Science,1995,21:261-269.
[9] Martin C,Smith A M.Starch biosynthesis[J].Plant Cell,1995,7:971-985.
[10] Okagaki R J,Wessler S R.Comparison of non-mutant and mutant waxy genes in rice and maize[J].Genetics,1988,120:1137-1143.
[11] Yamanouchi H,Nakamura Y.Organ specificity of isoforms of starch branching enzyme (Q-enzyme) in rice[J].Plant Cell Physiology,1992,33:985-991.
[12] Mizuno K,Kawasaki T,Shimada H,et al.Alteration of the structural properties of starch components by the lack of an isoform of starch branching enzyme in rice seeds[J].Journal of Biological Chemistry,1993,268:19084-19091.
[13] Han Y P,Xu M L,Liu X Y,et al.Gene coding for starching enzymes are major contributors to starch viscosity characteristics in waxy rice (Oryza sativa L.)[J].Plant Science,2004,166:357-364.
[14] 蔡秀玲,刘巧泉,汤述翥,等.用于筛选直链淀粉含量为中等的籼稻品种的分子标记[J].植物生理与分子生物学报,2002,28(2):137-144.
[15] 严长杰,田舜,张正球,等.水稻栽培品种淀粉合成相关基因来源及其对品质的影响[J].中国农业科学,2005,39(5):865-871.
[16] Nakamura Y.Towards a better understanding of the metabolic system for amylopectin biosynthesis in plants:rice endosperm as a model tissue[J].Plant Cell Physiology,2002,43(7):718-725.
[17] 吴洪恺,梁国华,顾燕娟,等.水稻淀粉合成相关基因对稻米RVA谱特征的影响[J].作物学报,2006,32(11):1597-1603.
[18] 陈峰,张正球,张士永,等.稻米淀粉的生物合成与品质改良的研究进展[J].山东农业科学,2008(3):20-25.
