In order to obtain drought-tolerant transgenic wheat lines,DNAj gene from foxtail millet,which was demonstrated to be related with drought and heat tolerant,was tried to introduce into 4 wheat cultivars via the pollen-tube pathway.The effects on seed setting under different pollination methods,different treatments with stigmas,DNA concentration and DNA diluents were studied and only the method of pollination showed distinct influence on the seed setting rate.Genomic PCR of T0 generation plants revealed that the DNAj gene fromfoxtail millet was integrated into one wheat plant by repeated assay.This indicated it was feasible to introduce DNAj gene fromfoxtail millet into wheat via the pollen-tube pathway even the transgenic efficiency was only 1. 42 ‰.Our work has established the foundation for creating drought-tol-erant wheat materials with foxtail millet genes.
WANG Yong-fang
,
ZHANGJun
,
CUI Run-li
,
LI Wei
,
ZHI Hui
,
LI Hai-quan
,
DIAO Xian-min
. Transformation of Wheat with DNAj Gene from Foxtail Millet via Pollen-tube Pathway[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(2)
: 17
-21
.
DOI: 10.7668/hbnxb.2009.02.004
[1] 郭京振,陈勇,檀书坤.重视农业水资源可持续利用问题[J].经济论坛,2003,24.
[2] 小麦干热风灾害等级[S].中华人民共和国气象行业标准.
[3] 崔润丽,智慧,王永芳,等.谷子DusJ蛋白基因的克隆[J].华北农学报,2007,22(4):9-13.
[4] 崔润丽,刁现民.植物耐盐相关基因克隆与转化研究进展[J].中国生物工程杂志.2005,25(8):25-29.
[6] 刘萍,张立杰,马宏玮,等.燕麦DNA导人普通小麦的初步研究[J].麦类作物学报,2000,20(4):6-11.
[7] 倪建福.高粱DNA导人小麦选育出抗条锈病白粒新品系[J].甘肃农业科技,1994(7):10-11.
[8] 欧巧明,倪建福,张正英,等.长穗偃麦草DNA导入引起的冬小麦后代性状变异及其遗传研究[J].麦类作物学报,2005,25(5):18-22.
[9] 崔良国,刘开昌.谷子DNA导人玉米创造变异及其利用的研究[J].山东农业科学,2002(3):18-20.
[10] 侯文胜,郭三堆,路明.利用花粉管通道法将cry Ia基因导入小麦[J].作物学报,2003,29(6):806-809.
[11] 周春江,葛荣朝,赵保存,等.利用花粉管通道法将兔防素基因NP-1导入小麦的研究[J].华北农学报,2007,22(2):26-28.
[12] 李兴林,王晓娟.外源DNA导入麦类作物结实率的研究[J].天津科技大学学报,2006,21(3):5-7,30.
[13] 黄景华,王广金,孙岩,等.小麦花粉管通道形成时期的研究[J].黑龙江农业科学,2004(2):20-22.
[14] Lewis Anahutika,Chawla H S.通过移液管将农杆菌注入小麦小穗中进行外源基因的转移时所涉及的因素的研究[J].Indian Journal of Genetics and Plant Breeding,2001,61(4):298-300.
[15] Zhu J K,Shi J,Bressan R A,et al.Expression of an gene encoding a protein homologous to the bacterial molecular chaperone DnaJ[J].Plant Cell,1993,5:341-349.
[16] 张玉秀,柴团耀李军超,等.DnaJ-like基因在不同环境胁迫下的表达研究[J].西北植物学报,2000,20(2):171-174.
[17] 柴团耀,张玉秀,Burkard G.莱豆重金属胁迫响应基因-cDNAs克隆及其表达分析[J].植物生理学报,1998,24:399-404.
