To clone and analyse mouse prolactin-releasing peptide. Total RNA was extracted from hypothalamus and amplified by RT-PCR. The PCR products were ligated into the pMD-19T vector, and then transformed into competent cells of E. coli DH5a, The positive cloning was sequenced and anelyzed.The cloned nucleotide sequence shared 86.3% hourology with the code zone sequence of rat prolactin-releasing peptide. The cloned nucleotide sequence was pert of mouse polactin-releasing peptide gene.
ZHU He-shui
,
DU Jun-xia
,
WANG Xin-wei
,
WANG Yue-ying
,
WANG Yan-ling
. Primary Cloning of Mouse Prolactin-releasing Peptide cDNA[J]. Acta Agriculturae Boreali-Sinica, 2007
, 22(6)
: 199
-200
.
DOI: 10.7668/hbnxb.2007.06.041
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