Study on Preparation Methods of Exserohilum turcicum for Intact Chromosome DNA

  • LIU Zhen-pan ,
  • WANG Yan-hui ,
  • DONG Jin-gao
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  • Mycotoxin Laboratory, Hebei Agricultural University, Baoding 071001, China

Received date: 2007-10-23

  Online published: 2014-10-14

Abstract

Several preparation methods for Exserolalum tureicum intact chromosome DNA were comparatively stuied, in order to establish an all-sided system to analysis the molecular karyotype of this fungi. The results showed that chromosome DNA of Exserohalum tungzaghubum were only obtained by the agarose-embedding protoplasts method, other methods gave incompetent results. Moreover, two chromosomal bands were separated by PFGE, both were more than 2200 kb.

Cite this article

LIU Zhen-pan , WANG Yan-hui , DONG Jin-gao . Study on Preparation Methods of Exserohilum turcicum for Intact Chromosome DNA[J]. Acta Agriculturae Boreali-Sinica, 2007 , 22(6) : 176 -178 . DOI: 10.7668/hbnxb.2007.06.036

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