Elymus canadensis, E.sibiricen and their Hybrid F1, F2 were analyzed by using random amplified poly-morphic DNA(RAPD)markers. The results showed that the two parents Elymus canadensis and E. sibiricus had relatively closer relationships. The RAPD bands of the hybrid F1 were mainly mutual supplement RAPD bands of the parents; at the same time, some RAPD bands of the parents were lost, the RAPD types of hybrid F1 showed a tendency toward the maternal type. The RAPD bands of F2 showed that the geneticrelationships between F2 and their parents were almost the same, but it also showed a tendency toward the maternal type a bit. There were differences about RAPD types between parents and their hybrids; RAPD technique cenhe used to identify hybrid and to detect the plantswith object traits.
LI Jing-huan
,
YUN Jin-feng
,
TAI Li-hua
,
WANG Shu-yan
,
LU Jian-jun
. RAPD Analysis of Elymus canadensis, E.sibiricus and Their Hybrid F1, F2[J]. Acta Agriculturae Boreali-Sinica, 2007
, 22(6)
: 77
-80
.
DOI: 10.7668/hbnxb.2007.06.016
[1] 周永红, 郑有良, 杨俊良, 等. 10种披碱草属植物的RAPD分析及系统学意义[J]. 植物分类学报, 1999, 37(5): 425-433.
[2] 张颖, 周永红, 张利, 等. 鹅观草属、披碱草属、猬草属和仲彬草属物种的RAMP分析及系统学意义[J]. 西北植物学报, 2005, 25(2): 368-375.
[3] 周永红, 郑有良, 杨俊良, 等. 鹅观草属、披碱草属、猬草属和仲彬草属植物的RAPD分析及系统学意义[J]. 四川农业大学学报, 2001, 19(1): 14-20.
[4] 王树彦. 加拿大披碱草与老芒麦种间杂种F1代的育性恢复研究. 博士学位论文[D]. 呼和浩特: 内蒙古农业大学, 2003.
[5] 韩冰. 针茅属植物基因组DNA提取方法的研究[J]. 内蒙古农业大学学报, 2002, 23(4): 32-35.
[6] 黄留玉. PCR最新技术原理、方法及应用[M]. 北京: 化学工业出版社, 2004: 261-269.
[7] Williams J G K, Kubelik A R, Livak K J, et al. DNApolymorphism amplified by arbitary primer are useful as genetic markers[J]. Nucleic Acid Res, 1990, 18: 6531-6535.
[8] 李造哲, 马青枝, 云锦凤, 等. 加拿大披碱草和老芒麦及其杂种F1同工酶分析[J]. 中国草地, 2000(5): 28-31.
[9] 马艳红, 于卓, 赵晓杰, 等. 加拿大披碱草-野大麦三倍体杂种加倍植株同工酶分析[J]. 草地学报, 2004, 12(2): 98-102.
