525 pairs of common wheat SSRs primers distributed in the total genomes of T. aestivum were used to identify the transferability in Thanopyrum intermedaum and to analyze the genetic relationship between T. aestivum Thinopyrum intermedaum.The results were、follows: 202 pairs SSRs primers amplified polymorphism between T.aestivum and Thancpyrum intermedium, which polymorphic percentage was 38.4%.And the polymorphic is percentage between T. aestivum on genome A, B, D and Thanopyrum intermedaum was 34.6%, 36.9% and 42.2% respectively.These resups showed that wheat SSRs pruners can be used in Tlanopyrum intermedaum; the genetic relationship between T. aestiwum on genome D and Thancpyrum intermedaum was far than that of on genome A, B, which indicated that therewas genetic differentiation within T. aestivum genome A, B, and D.
WANG Li-ming
,
LI Xing-feng
,
LIU Shu-bing
,
WANG Hong-gang
. Studies on the Transferability of Common Wheat(T. Aestivum) Microsatellites(SSR)Markers Used in Thinopyrum intermedium[J]. Acta Agriculturae Boreali-Sinica, 2007
, 22(6)
: 50
-52
.
DOI: 10.7668/hbnxb.2007.06.010
[1] 王黎明, 林小虎, 王洪刚, 等. 中间偃麦草的染色体组及其优良基因向普通小麦的转移[J]. 中国草地, 2005, 27(1): 57-63.
[2] 李永祥, 李斯深, 李立会, 等. 披碱草属12个物种遗传多样性的ISSR和SSR比较分析[J]. 中国农业科学, 2005, 38(8): 1522-1527.
[3] Sun G L, Salomon B, von Botluner R. Analysis of tetraploid Elymus species using wheat microsatellite markers and RAPD markers[J]. Genome, 1997, 40: 806-814.
[4] MacRitclue D, Sun G L. Evaluating the potential of barley and wheat microsatellite markers or genetic analysis of Elymus trachycaulus complex species[J]. Theor Appl Genet, 2004, 108: 720-724.
[5] Khlestkina E K, Myint Than M H, Pestsova E G, et al. Mapping of 99 new microsatellite-derived loci in rye(Secale cereale L.)including 39 expressed sequence tags[J]. Theor Appl Genet, 2004, 109: 725-732.
[6] 时津霞, 乔永利, 杨庆文, 等. 野生二粒小麦(Triticum dicoccoides)与普通小麦(T. aestivum)A、B染色体组的同源性分析[J]. 作物学报, 2005, 31(6): 723-729.
[7] 杨新泉, 刘鹏, 韩宗福, 等. 普通小麦、斯卑尔脱小麦和密穗小麦基因组中SSR和EST-SSR分子标记的遗传差异研究[J]. 自然科学进展, 2004, 14(9): 989-998.
[8] Roder M S, Korzun V. Wendehake K, et al. A microsatellite map of wheat[J]. Genetics, 1998, 149: 2007-2023.
[9] Gypta P K, Balyan H S, Edward K J, et al. Genetic mapping of 66 new microsatellite(SSR)in bread wheat[J]. Theor Appl Genet, 2002, 105: 413-422.
[10] Devos K M, Gale M D. The use of random amplified polymorphic DNA marker in wheat[J]. Theor Appl Genet, 1992, 84: 567-572.
[11] 李文才, 李涛, 赵逢涛, 等. 小麦D基因组产量性状QTL定位[J]. 华北农学报, 2005, 20(1): 23-26.