Molecular Cloning and Characteristics of cDNA Encoding Pig β1 Subunit for FMDV Receptor

  • GAO Shan-dian ,
  • DU Jun-zheng ,
  • CHANG Hui-yun ,
  • ZHOU Jian-hua ,
  • XIE Qing-ge
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  • Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, National FMD Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

Received date: 2007-09-25

  Online published: 2014-10-14

Abstract

In this study, we first molecularly cloned pig integrin β1 from RNA of the tongue and lung of recovered pig infected experunentally with foot-and-mouth-disease virus(FMDV), and compared with the β1 gene of other animals available in GenBank at nucleotide and amino acid loves. The 2397 bp cDNA of pig irnegrln β1 encodes a polypeptide of 798 amino acids consisting of 10 potential N-linked glycosylation sites(NXTX/NXSX), 2 EGF-like domains and 3 cys-teine-rich regions. Pig integnin β1 aubunit has a 20-residue putative signal peptide, a 708-residue ectodomain, a 29-residue transmembrane domain, and a 41-residue cytoplasmic domain.The nucleotide sequence similarity of integrin βl between bovine, chimpanzee, cat, dog, human, mouse, chicken is 99.5%, 90.0%, 91.8%, 90.7%, 90.2%, 86.5% and 77.4%, and the amino acid sequence similarity is 99.9%, 93.9%, 97.5%, 96.7%, 94.2%, 92.4% and 94. 9% respectively.Hydrophobicity analysis of the polypepticle revealed two hydrophobic domains, the signal peptide(1-20AA)and transmembrane domain(729-757AA).This study will lay a foundation for understarxling the interactions of FMDV with recptors.

Cite this article

GAO Shan-dian , DU Jun-zheng , CHANG Hui-yun , ZHOU Jian-hua , XIE Qing-ge . Molecular Cloning and Characteristics of cDNA Encoding Pig β1 Subunit for FMDV Receptor[J]. Acta Agriculturae Boreali-Sinica, 2007 , 22(6) : 14 -18 . DOI: 10.7668/hbnxb.2007.06.004

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