In this study, we first molecularly cloned pig integrin β1 from RNA of the tongue and lung of recovered pig infected experunentally with foot-and-mouth-disease virus(FMDV), and compared with the β1 gene of other animals available in GenBank at nucleotide and amino acid loves. The 2397 bp cDNA of pig irnegrln β1 encodes a polypeptide of 798 amino acids consisting of 10 potential N-linked glycosylation sites(NXTX/NXSX), 2 EGF-like domains and 3 cys-teine-rich regions. Pig integnin β1 aubunit has a 20-residue putative signal peptide, a 708-residue ectodomain, a 29-residue transmembrane domain, and a 41-residue cytoplasmic domain.The nucleotide sequence similarity of integrin βl between bovine, chimpanzee, cat, dog, human, mouse, chicken is 99.5%, 90.0%, 91.8%, 90.7%, 90.2%, 86.5% and 77.4%, and the amino acid sequence similarity is 99.9%, 93.9%, 97.5%, 96.7%, 94.2%, 92.4% and 94. 9% respectively.Hydrophobicity analysis of the polypepticle revealed two hydrophobic domains, the signal peptide(1-20AA)and transmembrane domain(729-757AA).This study will lay a foundation for understarxling the interactions of FMDV with recptors.
GAO Shan-dian
,
DU Jun-zheng
,
CHANG Hui-yun
,
ZHOU Jian-hua
,
XIE Qing-ge
. Molecular Cloning and Characteristics of cDNA Encoding Pig β1 Subunit for FMDV Receptor[J]. Acta Agriculturae Boreali-Sinica, 2007
, 22(6)
: 14
-18
.
DOI: 10.7668/hbnxb.2007.06.004
[1] Logan D, Abu-Ghazaleh R, Blakemore W, et al. Structure of a major immunogenic site on foot-and-mouth disease virus[J]. Nature, 1993, 362: 566-568.
[2] Jackson T, King A M, Stuart D I, et al. Structure and receptor binding[J]. Virus Res, 2003, 91: 33-46.
[3] Berinstein A, Roivainen M, Hovi T, et al. Antibodies to the vitronectin receptor(integrin αvβ3)inhibit binding and infection of foot-and-mouth disease virus to cultured cells[J]. J Virol, 1995, 69: 2664-2666.
[4] Jackson T, Sheppard D, Denyer M, et al. The epithelial integrin αvβ6 is a receptor for foot-and-mouth disease virus[J]. J Virol, 2000, 74: 4949-4956.
[5] Jackson T, Mould A P, Sheppard D, et al. Integrin αvβ1 is a receptor for foot-and-mouth disease virus[J]. J Virol, 2002, 76: 935-941.
[6] Jackson T, Clark S, Berryman S, et al. Integrin αvβ8 functions as a receptor for foot-and-mouth disease virus: Role of the β-chain cytodomain in integrin-mediated infection[J]. J Virol, 2004,(78)9: 4533-4540.
[7] Berryman S, Clark S, Monaghan P, et al. Early events in integrin αvβ6 -mediated cell entry of foot-and-mouth disease virus[J]. J Virol, 2005, 79: 8519-8534.
[8] Alexandesern S, Zhang Z, Donaldson A I, et al. The pathogenisis and diagnosis of foot-and-mouth disease[J]. J Comp Pathol, 2003, 129: 1-36.
[9] Spillmann D. Heperan sulfate: anchor for viral infection[J]. Biochumie, 2001, 83: 811-817.
[10] Fry E, Lea S M, Jackson T. The structure and function of a foot-and-mouth disease virus-oligosaccharide receptor complex[J]. EMBO, 1999, 18: 543-554.
[11] Ruoslahti E. RGD and other recognition sequences for integrins[J]. Annu Rev Cell Dev Biol, 1996, 12: 697-715.
[12] Duque H, Larocco M, Golde W T, et al. Interactions of foot-and-mouth disease virus with soluble bovine αvβ3 and αvβ6 integrin[J]. J Virol, 2004, 78: 9773-9781.
[13] Wickham T J, Mathias P, Cheresh D A, et al. Integrin alpha3beta1(CD 49c/29)is a cellular receptor for Kaposi′s sarcoma-associated herpesvirus(KSHV/HHV-8)entry into the target cells[J]. Cell, 2002, 108: 407-419.