Modeling and Analysis of 3C Protease Structure and Function from A Foot-and-month Disease Virus Strain OA/58

  • ZHOU Jian-hua ,
  • CONG Guo-zheng ,
  • GAO Shan-dian ,
  • CHANG Hui-yun
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  • Chinese Academy of Agriculture Sciences, Lanzhou Veterinary Research Insititute, State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National FMD Reference Laboratory, Lanzhou, 730046, China

Received date: 2007-09-24

  Online published: 2014-10-14

Abstract

Foot-and-mouth disease virus strain OA/58 RNAs were used as templates for RT-PCR.The amplified cDNA products were cloned into pGEM-T Easy Vectors and transformed into JM 109. The recombinant plasmids were identified by electrophoresis, PCR, and Eco R I cleavage. The nucleotide and amino acid sequences were compared with the 3C sequences of poliovirus, Hepatitis A virus and Human rhinovirus 89. The sequences alignments revealed that the agreement of OA/ 58 3C sequence to three reference viruses are 38.8%, 37. 1%, 36.5% at the nucleotide level, 23. 7%, 19. 2%, 17.6% at the acid level. By means of Swiss-pdbV iewer software, the 3D and surface mold of FMDV OA/58 3C protease was ohtained. In addition, Cys31-His46-Asp84 may he an active site in FMDV、OA/58 3C protease.

Key words: FMDV; 3C protease; Active site

Cite this article

ZHOU Jian-hua , CONG Guo-zheng , GAO Shan-dian , CHANG Hui-yun . Modeling and Analysis of 3C Protease Structure and Function from A Foot-and-month Disease Virus Strain OA/58[J]. Acta Agriculturae Boreali-Sinica, 2007 , 22(6) : 9 -13 . DOI: 10.7668/hbnxb.2007.06.003

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