Foot-and-mouth disease virus strain OA/58 RNAs were used as templates for RT-PCR.The amplified cDNA products were cloned into pGEM-T Easy Vectors and transformed into JM 109. The recombinant plasmids were identified by electrophoresis, PCR, and Eco R I cleavage. The nucleotide and amino acid sequences were compared with the 3C sequences of poliovirus, Hepatitis A virus and Human rhinovirus 89. The sequences alignments revealed that the agreement of OA/ 58 3C sequence to three reference viruses are 38.8%, 37. 1%, 36.5% at the nucleotide level, 23. 7%, 19. 2%, 17.6% at the acid level. By means of Swiss-pdbV iewer software, the 3D and surface mold of FMDV OA/58 3C protease was ohtained. In addition, Cys31-His46-Asp84 may he an active site in FMDV、OA/58 3C protease.
ZHOU Jian-hua
,
CONG Guo-zheng
,
GAO Shan-dian
,
CHANG Hui-yun
. Modeling and Analysis of 3C Protease Structure and Function from A Foot-and-month Disease Virus Strain OA/58[J]. Acta Agriculturae Boreali-Sinica, 2007
, 22(6)
: 9
-13
.
DOI: 10.7668/hbnxb.2007.06.003
[1] Grubman M J, Baxt B. Foot-and-mouth disease[J]. Clin Microbiol Rev, 2004, 17: 465-493.
[2] Knowles N J, Samuel P R. Molecular epidemiology of foot-and-mouth disease virus[J]. Virus, 2003, 91: 65-80.
[3] Mason P W, Grubman M J, Baxt B. Molecular basis of pathogenesis of FMDV[J]. Virus Res, 2003, 97(1): 9-32.
[4] Birtley J R, Knox S R, Jaulent A M, et al. Crystal structure of foot-and-mouth disease virus 3C protease: New insights into catalytic mechanism and cleavage specificity[J]. J Biol Chem, 2005, 280(12): 11520-11527.
[5] Belsham G J, McInerney G M, Ross S N. Foot-and-mouth disease virus 3C protease induces cleavage of translation initiation factors eIF4A and eIF4G within infected cell[J]. J Virol, 2000, 74: 272-280.
[6] 丁达夫, 汤海旭, 张保红, 等. 同源蛋白质三级结构的预测[M], 理论物理与生命科学. 上海: 上海科学技术出版社, 1997: 119-148.
[7] Birtley J R, Knox S R, Jaulent A M, et al. Crystal structure of foot-and-mouth disease virus 3C protease: New insights into catalytic mechanism and cleavage specificity[J]. J Biol Chem, 2005, 280(12): 11520-11527.