Identification and Pathogenicity Differentiation of the Pathogens Causing Necrotic Spot on Sunflower Broomrape

doi:10.7668/hbnxb.20192199

Abstract

Abstract:

In order to obtain the biocontrol strains of sunflower broomrape,this study took the occurrence of necrotic spot as samples of sunflower broomrape,and used the Koch's rule to separate and identify 29 samples of necrotic spot collected from 11 different locations in Inner Mongolia,Hebei and Xinjiang.The results showed that Fusariun oxysporum and F.solani were isolated from the disease samples collected from the 183 Regiment and 188 Regiment of Beitun Agricultural Tenth Division,Xinjiang and Xihaizi Village,Wuchuan County,Hohhot.F.oxysporum and F.equiseti were isolated from disease samples from Xixiaozhao Town,Urad Front Banner,Kuiboyuan,Wuyuan County and Yongli Village,Linhe District in Bayannur City.F.oxysporum,F.solani,F.equiseti and F.proliferatum were identified from the disease samples in the Gonghecheng Village and Xigedan Village,Siziwang Banner and a plot next to Provincial Highway 105 in Huade County.Only F.oxysporum was isolated from the samples collected in Dongsheng District,Ordos.In Kangbao County,Zhangjiakou City,Hebei Province,only F.verticillioides was isolated from samples collected.The above-mentioned isolated strains could cause disease symptoms after being re-inoculated on Helianthus angustifolia.The result indicated that the fungus of the genus Fusarium was the pathogenic fungi that caused blight in sunflower broomrape.However,F. oxysporum was the predominant pathogen of sunflower broomrape necrotic spot.The results of pathogenic differentiation studies showed that all the isolated strains were able to infect sunflower and broomrape,however,there were certain differences in their pathogenicity,which mainly exhibited stronger pathogenicity on isolated hosts than that of other host.Based on the results of pathogenicity differentiation,three strains of F.oxysporum GHC 1-2,XXZ-9 and HD 1-1 with biocontrol potential on broomrape were selected to control the parasitism of broomrape on sunflower.

Key words: Sunflower broomrape, Broomrape necrotic spot, Koch's rule, Isolation and identification of pathogenic fungi, Reciprocal pathogenicity

ZHANG Jian, WANG Na, LIU Zhida, BAO Tingting, ZHANG Zhiwei, YUN Xiaopeng, SHI Bixian, ZHANG Heng, CHEN Guihong, CHEN Yanfang, ZHAO Jun. Identification and Pathogenicity Differentiation of the Pathogens Causing Necrotic Spot on Sunflower Broomrape[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 172-180. doi: 10.7668/hbnxb.20192199.

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Figures/Tables 8

References 23

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样本编号 Sample number	采集地点 Gathering place	经纬度 Longitude and latitude	样本数量 Sample size
1	新疆北屯农十师183团	47°16'N,88°50'E	4
2	新疆北屯农十师188团	47°17'N,87°43'E	1
3	内蒙古巴彦淖尔市乌拉特前旗西小召镇	40°52'N,108°21'E	2
4	内蒙古巴彦淖尔市五原县葵博园	41°05'N,108°16'E	2
5	内蒙古巴彦淖尔市临河区永利村	41°08'N,107°30'E	1
6	内蒙古呼和浩特市武川县西海子村	41°12'N,110°45'E	8
7	内蒙古鄂尔多斯市东胜区	39°49'N,109°58'E	1
8	内蒙古乌兰察布市四子王旗公合成村	41°48'N,111°37'E	4
9	内蒙古乌兰察布市四子王旗西圪旦村	41°36'N,111°57'E	3
10	内蒙古乌兰察布市化德县105省道边	42°01'N,114°17'E	2
11	河北张家口市康保县	41°51'N,114°36'E	1

样本编号 Sample number	采集地点 Gathering place	经纬度 Longitude and latitude	样本数量 Sample size
1	新疆北屯农十师183团	47°16'N,88°50'E	4
2	新疆北屯农十师188团	47°17'N,87°43'E	1
3	内蒙古巴彦淖尔市乌拉特前旗西小召镇	40°52'N,108°21'E	2
4	内蒙古巴彦淖尔市五原县葵博园	41°05'N,108°16'E	2
5	内蒙古巴彦淖尔市临河区永利村	41°08'N,107°30'E	1
6	内蒙古呼和浩特市武川县西海子村	41°12'N,110°45'E	8
7	内蒙古鄂尔多斯市东胜区	39°49'N,109°58'E	1
8	内蒙古乌兰察布市四子王旗公合成村	41°48'N,111°37'E	4
9	内蒙古乌兰察布市四子王旗西圪旦村	41°36'N,111°57'E	3
10	内蒙古乌兰察布市化德县105省道边	42°01'N,114°17'E	2
11	河北张家口市康保县	41°51'N,114°36'E	1

菌株编号 Strain number	采集地点 Gathering place	比对结果 Blast result
183 1-1	新疆北屯农十师183团	尖孢镰刀菌
183 1-2	新疆北屯农十师183团	尖孢镰刀菌
183 2-1	新疆北屯农十师183团	尖孢镰刀菌
183 2-4	新疆北屯农十师183团	茄病镰刀菌
188 1-2	新疆北屯农十师188团	茄病镰刀菌
GHC 1-1	内蒙古乌兰察布市四子王旗公合成村	尖孢镰刀菌
GHC 1-2	内蒙古乌兰察布市四子王旗公合成村	尖孢镰刀菌
GHC 2-2	内蒙古乌兰察布市四子王旗公合成村	茄病镰刀菌
GHC 3-1	内蒙古乌兰察布市四子王旗公合成村	尖孢镰刀菌
XHZ	内蒙古呼和浩特市武川县西海子村	茄病镰刀菌
XHZ 1-1	内蒙古呼和浩特市武川县西海子村	茄病镰刀菌
XHZ 1-2	内蒙古呼和浩特市武川县西海子村	茄病镰刀菌
XHZ 1-7	内蒙古呼和浩特市武川县西海子村	茄病镰刀菌
XHZ 3-2	内蒙古呼和浩特市武川县西海子村	尖孢镰刀菌
XHZ-10	内蒙古呼和浩特市武川县西海子村	茄病镰刀菌
XHZ-15	内蒙古呼和浩特市武川县西海子村	尖孢镰刀菌
XHZG2	内蒙古呼和浩特市武川县西海子村	尖孢镰刀菌
XXZ-9	内蒙古巴彦淖尔市乌拉特前旗西小召镇	尖孢镰刀菌
XXZ-13	内蒙古巴彦淖尔市乌拉特前旗西小召镇	尖孢镰刀菌
XGD-1	内蒙古乌兰察布市四子王旗西圪旦村	层出镰刀菌
XGD 3-10	内蒙古乌兰察布市四子王旗西圪旦村	尖孢镰刀菌
XGD 3-13	内蒙古乌兰察布市四子王旗西圪旦村	木贼镰刀菌
WYKBY-2	内蒙古巴彦淖尔市五原县葵博园	木贼镰刀菌
WYKBY-5	内蒙古巴彦淖尔市五原县葵博园	尖孢镰刀菌
HD-2	内蒙古乌兰察布市化德县105省道边	尖孢镰刀菌
HD 1-1	内蒙古乌兰察布市化德县105省道边	尖孢镰刀菌
KBX-2	河北张家口市康保县	轮枝镰刀菌
YL-1	内蒙古巴彦淖尔市临河区永利村	木贼镰刀菌
DS-11	内蒙古鄂尔多斯市东胜区	尖孢镰刀菌

菌株编号 Strain number	采集地点 Gathering place	比对结果 Blast result
183 1-1	新疆北屯农十师183团	尖孢镰刀菌
183 1-2	新疆北屯农十师183团	尖孢镰刀菌
183 2-1	新疆北屯农十师183团	尖孢镰刀菌
183 2-4	新疆北屯农十师183团	茄病镰刀菌
188 1-2	新疆北屯农十师188团	茄病镰刀菌
GHC 1-1	内蒙古乌兰察布市四子王旗公合成村	尖孢镰刀菌
GHC 1-2	内蒙古乌兰察布市四子王旗公合成村	尖孢镰刀菌
GHC 2-2	内蒙古乌兰察布市四子王旗公合成村	茄病镰刀菌
GHC 3-1	内蒙古乌兰察布市四子王旗公合成村	尖孢镰刀菌
XHZ	内蒙古呼和浩特市武川县西海子村	茄病镰刀菌
XHZ 1-1	内蒙古呼和浩特市武川县西海子村	茄病镰刀菌
XHZ 1-2	内蒙古呼和浩特市武川县西海子村	茄病镰刀菌
XHZ 1-7	内蒙古呼和浩特市武川县西海子村	茄病镰刀菌
XHZ 3-2	内蒙古呼和浩特市武川县西海子村	尖孢镰刀菌
XHZ-10	内蒙古呼和浩特市武川县西海子村	茄病镰刀菌
XHZ-15	内蒙古呼和浩特市武川县西海子村	尖孢镰刀菌
XHZG2	内蒙古呼和浩特市武川县西海子村	尖孢镰刀菌
XXZ-9	内蒙古巴彦淖尔市乌拉特前旗西小召镇	尖孢镰刀菌
XXZ-13	内蒙古巴彦淖尔市乌拉特前旗西小召镇	尖孢镰刀菌
XGD-1	内蒙古乌兰察布市四子王旗西圪旦村	层出镰刀菌
XGD 3-10	内蒙古乌兰察布市四子王旗西圪旦村	尖孢镰刀菌
XGD 3-13	内蒙古乌兰察布市四子王旗西圪旦村	木贼镰刀菌
WYKBY-2	内蒙古巴彦淖尔市五原县葵博园	木贼镰刀菌
WYKBY-5	内蒙古巴彦淖尔市五原县葵博园	尖孢镰刀菌
HD-2	内蒙古乌兰察布市化德县105省道边	尖孢镰刀菌
HD 1-1	内蒙古乌兰察布市化德县105省道边	尖孢镰刀菌
KBX-2	河北张家口市康保县	轮枝镰刀菌
YL-1	内蒙古巴彦淖尔市临河区永利村	木贼镰刀菌
DS-11	内蒙古鄂尔多斯市东胜区	尖孢镰刀菌

菌株 Strains	分类地位 Taxonomic status	病斑长度 Lesion length		菌株 Strains	分类地位 Taxonomic status	病斑长度 Lesion length
菌株 Strains	分类地位 Taxonomic status	列当 Broomrape	向日葵 Sunflower	菌株 Strains	分类地位 Taxonomic status	列当 Broomrape	向日葵 Sunflower
183 1-1	尖孢镰刀菌	1.77±0.21fg	0.50±0.24efg	DS-11	尖孢镰刀菌	3.73±0.45bc	1.13±0.26bcd
183 1-2	尖孢镰刀菌	3.10±0.14cde	1.23±0.37bc	183 2-4	茄病镰刀菌	3.47±0.29bc	1.23±0.21bc
183 2-1	尖孢镰刀菌	3.43±0.45cd	0.63±0.25efg	188 1-2	茄病镰刀菌	2.53±0.37def	0.67±0.09efg
GHC 1-1	尖孢镰刀菌	2.90±0.24cde	0.67±0.26efg	GHC 2-2	茄病镰刀菌	2.90±0.43cd	0.47±0.09efg
GHC 1-2	尖孢镰刀菌	4.60±0.45a	0.40±0.22efg	XHZ	茄病镰刀菌	2.87±0.41def	0.37±0.09fg
GHC 3-1	尖孢镰刀菌	3.73±0.31bc	0.57±0.17efg	XHZ 1-1	茄病镰刀菌	2.73±0.46def	0.40±0.08efg
XHZ 3-2	尖孢镰刀菌	3.20±0.36cd	1.43±0.25b	XHZ 1-2	茄病镰刀菌	2.13±0.21fg	0.73±0.21def
XHZ-15	尖孢镰刀菌	1.53±0.38gh	0.30±0.14fg	XHZ 1-7	茄病镰刀菌	3.60±0.50bc	0.53±0.26efg
XHZG2	尖孢镰刀菌	3.33±0.40cd	0.37±0.17fg	XHZ-10	茄病镰刀菌	2.23±0.33efg	0.73±0.25def
XXZ-9	尖孢镰刀菌	4.57±0.49a	0.83±0.05cde	XGD 3-13	木贼镰刀菌	3.50±0.14h	0.47±0.09efg
XXZ-13	尖孢镰刀菌	2.43±0.33def	2.33±0.29a	WYKBY-2	木贼镰刀菌	0.83±0.29h	0.83±0.29cde
XGD 3-10	尖孢镰刀菌	1.07±0.19bc	0.47±0.17efg	YL-1	木贼镰刀菌	2.93±0.41def	0.37±0.09fg
WYKBY-5	尖孢镰刀菌	0.80±0.22h	0.57±0.33efg	KBX-2	轮枝镰刀菌	2.53±0.37def	0.30±0.12fg
HD-2	尖孢镰刀菌	1.10±0.29h	0.53±0.25efg	XGD-1	层出镰刀菌	1.90±0.61gh	0.67±0.16efg
HD 1-1	尖孢镰刀菌	4.17±0.31ab	0.23±0.05efg

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