Abstract: Optimal solid??state fermentat ion conditions were studied according to the sporulation quantity of Tricho??dermaviride isolate Tv04??2 by simple factor and orthogonal experiments.The most optimal medium was formulated as following: 250 mL erlenmeyer flask contained 20 g of equal quality of maize powder and wheat bran mixture, 0. 1 g FeSO₄,7 mL water and 4 mL inoculum.The fermentat ion process was at a fluctuant temperature of 25, 20 and 28 for 2 days,respectively. Under this optimal medium and fermentation process, the spore concentrat ion of 4×10⁹cfu/ g can be ob??tained, and which accelerated the production and applicat ion of Trichoderma viride strain with high antagonistic activity ofginseng root diseases.

Key words: Trichoderma viride, Solid fermentation, Sporulat ion quantity

CLC Number: 

• S476

• TQ920.6