Comparison Between 18S rRNA and β-actin mRNA as Internal Standard: Ontogenetic Expression of SGLT1 mRNA in Duodenum of Broiler Chicken

doi:10.3321/j.issn:1000-7091.2006.03.026

Abstract

Abstract: Beta-actin mRNA and 18S rRNA are widely employed as internal control genes,with the assumption that they are expressed constitutively to a similar degree under same experimental conditions.The objective of this study was to investigate the SGLT1(Sodium glucose cotransporter 1) mRNA ontogenetic expression in duodenum of broiler chicken(0-58 d) by a semi-quantitative RT-PCR,using beta-actin mRNA and 18S rRNA as internal standard respectively.In this study,we tested this assumption by assessment of the transcription of these two genes in chicken intestinal tissues using a semi-quantitative RT-PCR.The results indicate showed that the SGLT1 mRNA ontogenetic expression were consistent whether beta-actin mRNA or 18S rRNA was acted as internal control.The SGLT1 mRNA abundance in duodenum of chicken increased from day 2 to day 30,then started to decline from day 44,and increased again from day 58.The SGLT1 mRNA expression level on day 2 and day 44 were lower significantly than that on day 16(P<0.05) and day 30(P<0.05) respectively.And the SGLT1 mRNA abundance on day 58 was lower significantly than that on day 30(P<0.05).It suggested that both beta-actin mRNA and 18S rRNA could be used as internal standard for semi-quantitative analysis of transport carrier gene mRNA in intestinal ontogenesis of broiler chicken.

Key words: 18S rRNA, β-actinactin mRNA, Internal standard, SGLT1 mRNA, Broiler chicken

CLC Number:

S831

WANG Xiu-qi, TAN Hui-ze, SU Hai-lin, SONG Yu-zhen, FENG Ding-yuan. Comparison Between 18S rRNA and β-actin mRNA as Internal Standard: Ontogenetic Expression of SGLT1 mRNA in Duodenum of Broiler Chicken[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(3): 112-116. doi: 10.3321/j.issn:1000-7091.2006.03.026.

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