Abstract:
The milk-clotting properties of kid rennet were investigated in the paper.The results showed that the optimum clotting temperature of kid rennet was 50℃.The milk-clotting activity of rennet was stable at less than 40℃,after 10 min treatment at 60℃,it would completely lose.The milk-clotting activity decreased with the increase of milk pHvalue between 5.5 and 7.5,at pH7.5 it almost losed completely.Treating kid rennet for 20 hours at the pHin the range of 2.0~7.0,the clotting activity maintained stable.Al3+,Ca2+and Na+ had obvious functions to accelerate milk coagulation.K+,Mg2+,Mn2+and Fe3+had no obvious functions to accelerate or inhibit milk coagulation.But Cu2+ and Zn2+ could keep milk from coagulating.The influence of milk concentration on enzymatic activity was also studied,obtaining the Km of kid rennet was 1.893g/L.
Key words:
Kid rennet,
Milk-clotting activity
CLC Number:
XU Ming-sheng, WANG Bi-ni, ZHANG Fu-xin. Investigation on the Milk-clotting Properties of Kid Rennet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(6): 103-106. doi: 10.3321/j.issn:1000-7091.2005.06.026.