Acta Agriculturae Boreali-Sinica ›› 2022, Vol. 37 ›› Issue (5): 213-221. doi: 10.7668/hbnxb.20193038

• Animal Husbandry·Fisheries·Veterinarian • Previous Articles     Next Articles

The Distribution and Expression Patterns of KISS1 and TrkA in Reproductive Axis of Pregnant Oryctolagus cuniculus f. domestica

AN Xiaoxiao1,2, ZHANG Bohao2,3, DAI Lijun1,2, BAI Xu1,2, SHI Jun1,2, ZHANG Xia1,2, ZHANG Yong1,2,3, ZHANG Quanwei1,2, ZHAO Xingxu1,2,3,   

  1. 1 College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China
    2 Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation,Lanzhou 730070,China
    3 College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China
  • Received:2022-06-01 Published:2022-10-28
  • Contact: ZHAO Xingxu


In order to clarify the expression pattern and physiological function of Kiss element (KISS1)and Tyrosine protein kinase A(TrkA) in the reproductive axis of ovulation inducing animals,and analyze the regulatory mechanism of KISS1 and TrkA on the reproduction of ovulation inducing animals.Domesticated rabbits were taken as an ovulation-inducing model.The reproductive axis tissues including hypothalamus,pituitary,ovary and uterus were collected from the pregnant Oryctolagus cuniculus f. domestica(Gestation lasts about 14—18 days).The expression patterns and distribution of KISS1 and TrkA mRNA and proteins was detected using hematoxylin-eosin(H&E)and immunohistochemistry(IHC)staining,semi-quantitative interpretation(RT-PCR),Real-time fluorescent quantitative PCR (qRT-PCR)and Western Blot(WB)in the HPG tissues.The results of IHC showed strongly positive reaction of KISS1 protein was presented in the pituitary basophils and zona pellucida of ovary while the positive expression of TrkA protein presented in the neuroendocrine cells of hypothalamus,basophils of pituitary,granular lutein cell of ovary and lamina propria of uterus.The results of RT-PCR KISS1 was expressed in the reproductive axis of pregnant rabbits,and there was significant difference between the two groups.The expression abundance of TrkA mRNA in hypothalamus,pituitary and ovary was significantly different between the two groups.The qRT-PCR results of KISS1 and TrkA genes were consistent with the RT-PCR results.The results of WB suggested that the relative expression levels of KISS1 protein was only observed in pituitary tissue while the relative expression levels of TrkA protein was presented in the HPG tissues and with a down-regulated trend.The results indicating that KISS1 and TrkA are participated in the regulation of pregnancy in Oryctolagus cuniculus f. domestica,and TrkA has a wider scope of action.

Keywords: Pregnant Oryctolagus cuniculus f. domestica;Reproductive axis;Reproductive hormone;Tyrosine protein kinase A(TrkA);Kiss element(KISS1)

Cite this article

AN Xiaoxiao, ZHANG Bohao, DAI Lijun, BAI Xu, SHI Jun, ZHANG Xia, ZHANG Yong, ZHANG Quanwei, ZHAO Xingxu. The Distribution and Expression Patterns of KISS1 and TrkA in Reproductive Axis of Pregnant Oryctolagus cuniculus f. domestica[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 213-221. doi: 10.7668/hbnxb.20193038.

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