The Role of 1-Aminocyclopropane-1-Carboxylate in the Chemotactic Response of Pseudomonas putida to Agaricus bisporus Mycelia

doi:10.7668/hbnxb.20190496

Abstract

Abstract: The fungal-bacterial biofilm (FBB) has been considered to play crucial roles in preparing high efficient biofertilizer inoculants and biocontrol agents. However, large scale preparation of FBB is time-consuming and difficult. It is essential to explore the mechanism of FBB forming. The role of 1-aminocyclopropane-1-carboxylate (ACC) on forming Agaricus bisporus -bacterial biofilm was studied by using A.bisporus and Pseudomonas putida UW4. On PDA plates, the mycelial growth rate of A.bisporus with UW4 suspension spreaded on edges of the mycelia improved 10.85%, compared to that with ultrapure water. Furthermore, the biofilm formed by UW4 on A.bisporus mycelial surface could be detected by microscopic examination and scanning electron microscope obserbation. In contrast, both UW4 and its ACC deaminase deficient mutant could not form biofilm on the surface of A.bisporus mycelia treated with the aminooxyacetic acid (AOA), the ACC synthase inhibitor. Moreover, the results of chemotactic assays showed UW4 was chemotactic towards ACC, and the chemotactic response of UW4 to ACC was similar to that of the glutamine and citric acid secreted by A.bisporus mycelia. The chemotactic ability of UW4 to ACC on the optimum ACC concentration was significantly higher than that of glutamine and citric acid, whereas the UW4-AcdS ^- showed absence of chemotactic towards ACC. In addition, the transcriptome sequencing analysis revealed that ACC could induce the up-regulation or down-regualtion of chemotactic-and movement-related genes of UW4. Taken together, ACC is a strong chemoattractant for the chemotactic response of P.putida to A. bisporus, and is the critical signaling substance for AcdS-producing bacteria forming biofilm on the fungal mycelial surface.

Key words: Fungal-bacterial biofilm, Agaricus bisporus, Pseudomonas, 1-aminocyclopropane-1-carboxylate, Chemoattractant

CLC Number:

S182
S646.1

ZHANG Chaohui, ZHANG Guang, WEN Yamei, YANG Yanqing, LIU Yafei, ZHANG Hui, WANG Zhenhe, QIU Liyou. The Role of 1-Aminocyclopropane-1-Carboxylate in the Chemotactic Response of Pseudomonas putida to Agaricus bisporus Mycelia[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 209-218. doi: 10.7668/hbnxb.20190496.

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