Abstract: The purpose of this study is to obtain mutant strain with high yields of γ-PGA and determine polyglutamic acid effect on calcium absorption in mice.Bacillus natto was irradiated by ultraviolet to induce mutation.The mutant strains were selected according to morphological changes.Then,the mutant strains were cultivated.The γ-poly glutamic acid (γ-PGA) of different mutant strains were separated and purified to determine its production.The mutant strain which had high yields of γ-PGA was selected and determined its genetic stability.The effects of the calcium solubility by γ-PGA in vitro and in vivo were measured as well as the bone mineral content in mice.Experiment screened out a mutant strain,Z-29,with stable high yields of γ-PGA greater than 18 g/L.The experimental results showed that γ-PGA promoted calcium solubility in vitro and in mice small intestine.Moreover,it increased bone calcium and bone phosphorus content in mice.This experiment gained Bacillus natto mutant strains with high yields of γ-PGA and proved that γ-PGA can increase the solubility of calcium in vitro and in vivo.It provided the basis for further research and application in production.

Key words: Polyglutamate, Calcium mice, Mice, Absorption

CLC Number: 

• Q78