ACTA AGRICULTURAE BOREALI-SINICA ›› 2011, Vol. 26 ›› Issue (4): 141-146. doi: 10.7668/hbnxb.2011.04.025

Special Issue: Oil crops

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Study on Actinomycete against Fusarium oxysporum f. sp. lini in Linseed

GUO Jing-xu1,2, LI Zi-qin2, ZHANG Hui2, BAO Yu-ying1   

  1. 1. Inner Mongolia University, Huhhot 010020, China;
    2. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Huhhot 010031, China
  • Received:2011-01-11 Published:2011-08-28

Abstract: In order to get a antagonistic actinomycete against linseed wilt,an actinomycete GS2-1 was isolated from pathogenic soil of linseed,which could inhibit growth of Fusarium oxysporum f. sp. lini. The inhibition rates against 151 fungi isolated from different provinces were evaluated. The fermentation filtrate influencing the mycelium growth and the spore bourgeoning and the optimum fermentation conditions were also conducted. Antagonistic substance was extracted from the fermentation liquid,its activity in lab and antagonistic effects against linseed wilt in control environment were tested. The results showed that GS2-1 had a strong antagonistic effect on those 151 fungi, the highest inhibition rate was 73. 9%. Antagonistic substance of GS2-1 appeared to inhibit pathogens by leading to mycelium rupture,distortion,abnormality,and inhibition bourgeon of spores,and leading to abnormality of spores. And the best fermentation time of GS2-1 was 6 days,the optimum temperature was 29 - 32℃,beginning pH 7. 2,and the optimal pH 7 - 9. The best medium was soybean farina medium (modified) and its volume of 100 mL per 300 mL flask. The antagonistic substance could be extracted by chloroform and has nice thermal stability. The control effect of GS2-1 was as high as 68. 8% in control environment.

Key words: Linseed wilt, Antagonistic actinomycete, Control effect

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Cite this article

GUO Jing-xu, LI Zi-qin, ZHANG Hui, BAO Yu-ying. Study on Actinomycete against Fusarium oxysporum f. sp. lini in Linseed[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 141-146. doi: 10.7668/hbnxb.2011.04.025.

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