Abstract: To investigate the effect of cell wall components of L. casei LC2W on the function of murine macrophagecells in vitro.The energy metabolism level, the phagocytic ability to neutral red of murine macrophage RAW264. 7 cellsand the content of NO derived from the cells were detected with different levels of teichoic acid ( TA) or pept idoglycan( PG), two predominant components of the cell wall of the cell L. casei LC2W, compared with those macrophage cells cu-ltured without TA or PG. The energy metabolism level, the phagocytic act ivity were significant ly hig her than those in controlgroup, and were also in a dosage-dependent manner, no obvious difference existed in RAW264. 7 cells challenged with Lactobacillus TA and those cells challenged with PG. The content of NO derived from murine macrophage RAW264. 7 cells macrophages induced by different levels of TA or PG was enhanced and when its 50 g/ mL, the content of NO induced by TA is higer than that by PG.TA and PG of the cell wall of L. casei LC2W could activate macrophage RAW264.7 cells to secrete active factors with tumoricidal activity, and enhance its energy metabolism level, phagocytic activity to neutral red signif icantly in a dosage-dependent manner.

Key words: L. casei LC2W, RAW264. 7 macrophag activity, Cell wall components

CLC Number: 

• Q2