Interspecific Somatic Hybrids Between Brassica oleracea var botrytis and Ogura TypecMS Brassica napus Obtained from Asymmetric Protoplast Fusion

doi:10.3321/j.issn:1000-7091.2006.03.016

Abstract

Abstract: Asymmetric protoplast fusion beween hypocotyl protoplasts of Brassica oleracea var.botrytis and leaf protoplasts of Ogura typecMS Brassica napus was performed by PEG method.Leaf protoplasts from donor plant were treated with UV light at a dose of 0.0250,0.075 0,0.315 0,0.4680 and 0.9600J/cm² respectively.68 plants regenerated in the dose range from 0.0250 to 0.4680J/cm²,and none at the dose of 0.9600J/cm².Results of chromosome counts,ploidy analysis by flow cytometry,isozyme analysis,RAPD and specific amplification(STS primers) of Ogura-CMS-associated mitochondrial gene orF₁38 revealed that 32 somatic hybrid plants were obtained.It was showed in our experiment that the degree of elimination of UV-irradiated donor chromosomes did not depend on the irradiation dose.

Key words: Brassica oleracea var.botrytis, Asymmetric protoplast fusion, OgucMS Brassica napus

CLC Number:

S603.6

HUI Zhi-ming, LIU Fan, JIAN Yuan-cai, SHEN Shu-xing, ZHAO Hong. Interspecific Somatic Hybrids Between Brassica oleracea var botrytis and Ogura TypecMS Brassica napus Obtained from Asymmetric Protoplast Fusion[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(3): 65-70. doi: 10.3321/j.issn:1000-7091.2006.03.016.

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