Abstract: An excretory-secretory(ES)antigen and an enzyme conjugate were used in a serodiagnostic enzyme-linked immunosorbent assay(ELTSA)for swine trichinosis. The antigen derived from shortterm in vitro maintenance of Trichinella spiralis muscle larvae was developed by modifying the cultivation technique to them on the basis of previous researches of Gamble et al. So the status quo applying crude antigen for diagnosing animal trichinosis in China may be changed from now on. All 71 Sera from pigs infected experimentally with T. spiralis were detected(detecting rate to positives.100%). In field tests on 120000hogs, the detecting rate to naturally-infected cases was 93-95%. Compared with the digestion technique by killing 250 ELISA positive swines the agreement rate of the test was around 98%. The study indicates that the test is sensitive, specific, and easy to perform. The result is also easy to reed.

Key words: Swine trichinosis, Excretory-Secretory antigen, HRP-SPA-ELISA