Cloning and Sequence Analysis of S and ORF3 Genes of Porcineepidemic diarrhea virus in Henan Province
ZHAO Li1, HAN Haoying2,3, ZHANG Hongxin2,3, LU Tingting1, WANG Wenjing1, CHEN Hongying2,3
1. Henan University of Animal Husbandry and Economy, Zhengzhou 450011, China;
2. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China;
3. Zhengzhou Major Pig Disease Prevention and Control Laboratory, Zhengzhou 450002, China
Abstract：In order to analyze the S and ORF3 genes of PEDV in Henan Province, 150 PED suspected diseases were isolated from different pig farms in Henan Province from January to December, 2015, and the sequences of S and ORF3 genes were analyzed. The result showed that the nucleotide and amino acid homologies of S genes from 15 strains with CV777 were 93.6%-93.9% and 92.2%-93.1%, respectively. And some base insertions and deletions were detected. Compared with CV777, the nucleotide homology of ORF3 gene was 97.7%-100% and the amino acid homology was 93.7%-100%. Additionally, there was no base insertion and deletion compared with the vaccine strain. The Phylogenetic tree was based on the 15 PEDV of S gene amplified and the relationship of these strains were relatively distant from other reference strains. The relationships of the 15 strains, amplified based on the ORF3 gene, were closely related to domestic isolates, American strains and Korean strains. For the entire evolutionary relationship of S and ORF3 genes, 15 Henan strains in this experiment were relatively independent and their relationships with classic strain CV777 and other vaccine strains used in China were distant.The experiment analyzed of the S and ORF3 gene sequence features in prevailing of Henan region, which provided a support of molecular epidemiology study and prevention and control of PED in Henan Province.
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2018, Vol. 33 
