接种丛枝菌根真菌增强药用牡丹的耐旱性

doi:10.7668/hbnxb.20196636

华北农学报 ›› 2026, Vol. 41 ›› Issue (4): 140-151. doi: 10.7668/hbnxb.20196636

所属专题： 抗旱节水；植物保护；栽培生理；

• 耕作栽培·生理生化 • 上一篇下一篇

接种丛枝菌根真菌增强药用牡丹的耐旱性

陈轲¹, 张彪¹, 张有福¹, 王梓², 龚明贵³, 常青山²^,⁴, 孙一鸣², 张利霞¹, 侯小改¹

¹ 河南科技大学农学院, 河南洛阳 471023
² 河南科技大学园艺与植物保护学院, 河南洛阳 471023
³ 河南科技大学食品与生物工程学院, 河南洛阳 471023
⁴ 河南省生态经济型木本植物种质创新与利用重点实验室, 河南洛阳 471023

收稿日期:2025-11-26 出版日期:2026-08-28
通讯作者:
张利霞(1982—),女,河南郑州人,副教授,博士,硕士生导师,主要从事药用植物抗逆研究。
侯小改(1966—),女,河南焦作人,教授,博士,博士生导师,主要从事牡丹研究。
作者简介:
陈轲(2001—),男,河南新乡人,在读硕士,主要从事药用植物抗逆研究。
基金资助:
国家自然科学基金项目(31800096); 河南省自然科学基金项目(242300420144); 河南省自然科学基金项目(252300423035); 河南省科技攻关项目(252102110148); 河南省中药材产业技术体系(HARS-22-11-S)

Inoculation with Arbuscular Mycorrhizal Fungi Enhances Drought Tolerance in Medicinal Tree Peony

CHEN Ke¹, ZHANG Biao¹, ZHANG Youfu¹, WANG Zi², GONG Minggui³, CHANG Qingshan²^,⁴, SUN Yiming², ZHANG Lixia¹, HOU Xiaogai¹

¹ College of Agriculture, Henan University of Science and Technology, Luoyang 471023, China
² College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China
³ College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
⁴ Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-economic Woody Plant, Luoyang 471023, China

Received:2025-11-26 Published:2026-08-28

语音导读.mp3(715KB)

摘要/Abstract

摘要：

探讨接种丛枝菌根真菌(AMF)对干旱胁迫下药用牡丹凤丹生理特性及活性成分积累的影响,为利用AMF增强药用牡丹抗旱能力及提升其药材品质提供理论依据。以凤丹实生苗为试验材料,设置正常水分(CK)、干旱胁迫(DR)、正常水分下接种AMF(AMCK)及干旱胁迫下接种AMF(AMDR)共4个处理,分别测定植株氧化还原稳态(包括超氧化物歧化酶(SOD)活性、脯氨酸和可溶性糖含量)、光合特性(包括光合色素含量、光合与叶绿素荧光参数)以及根皮中活性成分(总黄酮、丹皮酚、芍药苷等)含量。结果表明,干旱胁迫导致凤丹植株体内丙二醛(MDA)和活性氧(ROS)含量分别显著增加了33.53%和50.04%,光合性能受到明显抑制,具体表现为净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、最大光化学效率(Fv/Fm)和光合性能指数(PIabs)均显著下降。接种AMF有效缓解上述干旱胁迫引起的不利影响:不仅使SOD活性显著提高6.36%,可溶性蛋白、可溶性糖及脯氨酸等渗透调节物质含量分别显著提升23.72%,53.31%和14.70%,并降低活性氧含量,增强细胞膜稳定性,还使光合色素含量以及Pn、Gs、Tr、Fv/Fm、PIabs等光合荧光相关参数得到显著恢复。此外,在干旱胁迫条件下接种AMF进一步促进了根皮中总黄酮、丹皮酚和芍药苷等次生代谢产物的积累,三者含量较DR处理分别提升18.67%,11.39%和5.17%。综上,AMF能够通过增强凤丹的抗氧化能力、促进渗透调节物质积累、改善光合性能,有效缓解干旱胁迫对植株造成的生理损伤,同时提高根皮中次生代谢物质的含量。

关键词: 药用牡丹, 丛枝菌根真菌, 干旱胁迫, 光合特性, 活性成分, 抗氧化能力

Abstract:

This study investigated the effects of arbuscular mycorrhizal fungi(AMF)inoculation on the physiological characteristics and the accumulation of active components in the medicinal tree peony Paeonia ostii Fengdan under drought stress,with the aim of providing a theoretical basis for utilizing AMF to enhance the drought resistance and medicinal quality of this species.Using P.ostii seedlings as experimental material,four treatments were established:well-watered control(CK),drought stress(DR),AMF inoculation under well-watered conditions(AMCK),and AMF inoculation under drought stress(AMDR).Indicators related to plant redox homeostasis(including superoxide dismutase(SOD)activity,proline and soluble sugar content),photosynthetic characteristics(including photosynthetic pigment content,gas exchange parameters,and chlorophyll fluorescence parameters),and the content of active components(such as total flavonoids,paeonol,and paeoniflorin)in the root bark were measured.The results showed that drought stress induced significant accumulation of malondialdehyde(MDA)and reactive oxygen species(ROS),with increases of 33.53% and 50.04%, respectively,while photosynthetic performance was markedly inhibited.This was specifically evidenced by significant decreases in net photosynthetic rate(Pn),stomatal conductance(Gs),transpiration rate(Tr),maximum photochemical efficiency(Fv/Fm),and photosynthetic performance index(PIabs).AMF inoculation effectively alleviated these adverse effects.It was found that inoculation not only significantly increased SOD activity by 6.36% and elevated the contents of osmoregulatory substances (soluble proteins, soluble sugars, and proline) by 23.72%, 53.31%, and 14.70%, respectively,but also reduced ROS levels,thereby enhancing cell membrane stability.A significant recovery was also observed in photosynthetic pigment content and photosynthetic and chlorophyll fluorescence parameters(Pn,Gs,Tr,Fv/Fm,and PIabs).Furthermore,under drought stress conditions,AMF inoculation further promoted the accumulation of secondary metabolites in the root bark,including total flavonoids,paeonol,and paeoniflorin,with increases of 18.67%, 11.39%, and 5.17%, respectively, compared with the DR treatment.In conclusion,it is demonstrated that AMF can effectively mitigate the physiological damage caused by drought stress in P.ostii by enhancing its antioxidant capacity,promoting the accumulation of osmoregulatory substances,and improving photosynthetic performance.Simultaneously,AMF boosts the content of secondary metabolites in the root bark.

Key words: Medicinal peony, Arbuscular mycorrhizal fungi, Drought stress, Photosynthetic characteristics, Active components, Antioxidant capacity

中图分类号:

S318其他栽培技术与方法

陈轲, 张彪, 张有福, 王梓, 龚明贵, 常青山, 孙一鸣, 张利霞, 侯小改. 接种丛枝菌根真菌增强药用牡丹的耐旱性[J]. 华北农学报, 2026, 41(4): 140-151. doi: 10.7668/hbnxb.20196636.

CHEN Ke, ZHANG Biao, ZHANG Youfu, WANG Zi, GONG Minggui, CHANG Qingshan, SUN Yiming, ZHANG Lixia, HOU Xiaogai. Inoculation with Arbuscular Mycorrhizal Fungi Enhances Drought Tolerance in Medicinal Tree Peony[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(4): 140-151. doi: 10.7668/hbnxb.20196636.

http://www.hbnxb.net/CN/Y2026/V41/I4/140

图/表 9

表1 不同处理AMF侵染率

Tab.1 AMF colonization rate under different treatments

处理 Treatment	AMF侵染率/% AMF colonization rate
CK	18.52±4.49c
DR	10.74±1.70d
AMCK	81.11±1.11a
AMDR	60.37±2.31b

图1 AMF对干旱胁迫下凤丹叶片SOD活性、可溶性蛋白、可溶性糖与脯氨酸含量的影响不同小写字母表示各处理间差异显著(P<0.05)。图2—7同。

Fig.1 Effects of AMF on SOD activity and the contents of soluble protein,soluble sugar and proline in P. ostii leaves under drought stress Different lowercase letters indicate significant differences between different treatments(P<0.05).The same as Fig.2—7.

图2 AMF对干旱胁迫下凤丹叶片相对电导率、MDA、H₂O₂与$\mathrm{O}^{\bar{.}}_{2}$含量的影响

Fig.2 Effects of AMF on relative electrical conductivity and the contents of MDA,H₂O₂ and $\mathrm{O}^{\bar{.}}_{2}$ in P. ostii leaves under drought stress

图3 AMF对干旱胁迫下凤丹叶片中光合色素含量的影响

Fig.3 Effects of AMF on the content of photosynthetic pigments in P. ostii leaves under drought stress

表2 AMF对干旱胁迫下凤丹叶片气体交换参数的影响

Tab.2 Effects of AMF on gas exchange parameters in P.ostii leaves under drought stress

处理 Treatment	Pn/ (μmol/(m²·s))	Gs/ (mol/(m²·s))	Ci/ (μmol/mol)	Tr/ (mmol/(m²·s))
CK	6.63±0.12b	0.048±0.001c	155.93±2.37c	1.47±0.04b
DR	4.63±0.08d	0.041±0.001d	192.50±3.09a	1.38±0.02c
AMCK	7.45±0.04a	0.052±0.001a	142.88±2.78d	1.56±0.02a
AMDR	6.21±0.04c	0.050±0.000b	176.48±0.85b	1.52±0.01ab
干旱 Drought stress	^**	^**	^**	^**
AMF	^**	^**	^**	^**
干旱Drought stress×AMF	^**	^**	NS	NS

图4 AMF对干旱胁迫下凤丹叶片W_K、V_J、M₀、φE₀的影响

Fig.4 Effects of AMF on W_K,V_J,M₀,and φE₀ in P.ostii leaves under drought stress

图5 AMF对干旱胁迫下凤丹叶片Fv/Fm和PIabs的影响

Fig.5 Effects of AMF on Fv/Fm and PIabs in P.ostii leaves under drought stress

图6 AMF对干旱胁迫下凤丹根皮中总黄酮含量的影响

Fig.6 Effects of AMF on the content of total flavonoids in P.ostii root bark under drought stress

图7 AMF对干旱胁迫下凤丹根皮中活性成分含量的影响

Fig.7 Effects of AMF on the contents of active components in P.ostii root bark under drought stress

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