红麻始花节位QTL定位及候选基因分析

doi:10.7668/hbnxb.20196050

摘要/Abstract

摘要：

为了探明红麻始花节位的遗传规律,以始花节位差异显著的红麻自交系K215和K89为亲本,构建了含有148个单株的F₂群体。利用RAD-seq测序的方法进行bin标记基因分型,构建了红麻高密度遗传连锁图谱。该图谱含有3 418个bin标记且不均匀地分布在18条染色体上,图谱总遗传距离为864.61 cM,标记间平均遗传距离为0.25 cM。结合始花节位的表型数据和遗传图谱,利用区间作图法,检测到1个与始花节位密切相关的QTL位点FFN6。该位点位于第6号染色体,LOD值为8.66,表型贡献率为13.77%,加性效应值为-19.97。结果表明,该等位基因位点来自父本K215,对始花节位具有负调控作用。根据QTL定位区间内基因注释信息,初步筛选获得7个候选基因。qRT-PCR结果表明,短日照条件下Hc-bHLH6 和 Hc-MYB6基因的表达量显著增加,促进红麻植株开花,这2个候选基因可能与红麻植株始花节位密切相关。

关键词: 红麻, QTL, 始花节位, 遗传连锁图谱

Abstract:

In order to determine the genetic law of the first flower node in kenaf,an F₂ population consisting of 148 individual plants was developed from the inbred lines K215 and K89,which showed significant differences in the first flower node position.The high-density genetic linkage map of kenaf was constructed using RAD-seq for bin marker genotyping.The map contained 3 418 bin markers unevenly distributed on 18 chromosomes,with a total genetic distance of 864.61 cM and an average genetic distance between markers of 0.25 cM.Based on the phenotypic data and genetic map of the first flower node,the first flower node QTL named FFN6 was detected using an interval mapping method.This QTL was located on chromosome 6,with a LOD value of 8.66,a phenotypic contribution rate of 13.77%,and an additive effect value of -19.97.The results indicated that the allele locus comes from the paternal K215 and had a negative regulatory effect on the first flower node.Based on the gene annotation information within the QTL mapping interval,seven candidate genes were preliminarily screened and obtained.The qRT-PCR results showed that under short-day conditions,the expression levels of Hc-bHLH6 and Hc-MYB6 genes significantly increased.The function of the two genes promotes the flowering of kenaf plants,which may be closely related to the node of the first flower of kenaf plants.

Key words: Kenaf, QTL, First flower node, Genetic linkage map

中图分类号:

李辉, 陈安国, 唐慧娟, 栾明宝. 红麻始花节位QTL定位及候选基因分析[J]. 华北农学报, 2026, 41(3): 75-81. doi: 10.7668/hbnxb.20196050.

LI Hui, CHEN Anguo, TANG Huijuan, LUAN Mingbao. QTL Mapping and Candidate Gene Analysis of the First Flower Node in Kenaf[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(3): 75-81. doi: 10.7668/hbnxb.20196050.

http://www.hbnxb.net/CN/Y2026/V41/I3/75

图/表 6

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引物名称 Primer name	正向序列(5'-3') Forward sequence(5'-3')	反向序列(5'-3') Reverse sequence(5'-3')
bHLH	GCAGTTCCTCTCAATGAAGTTAGC	GATTTTGCTGGGACTGGTGC
MYB	GGGAAGAACAGATAATGAGGTGATG	GTCGGTGGGGTTAGGAGGAA
Hc-actin	TTGCAGACCGTATGAGCAAG	ATCCTCCGATCCAGACACTG

引物名称 Primer name	正向序列(5'-3') Forward sequence(5'-3')	反向序列(5'-3') Reverse sequence(5'-3')
bHLH	GCAGTTCCTCTCAATGAAGTTAGC	GATTTTGCTGGGACTGGTGC
MYB	GGGAAGAACAGATAATGAGGTGATG	GTCGGTGGGGTTAGGAGGAA
Hc-actin	TTGCAGACCGTATGAGCAAG	ATCCTCCGATCCAGACACTG

染色体 Chromosome	SNP 标记数量 No. of SNP marker	bin 标记数量 No. of bin marker	连锁群长度/cM Linkage distance	平均遗传距离/cM Average inter-loci distance	图谱空隙/cM Map gap
LG1	516	126	75.56	0.60	10.59
LG2	3 275	707	119.16	0.17	7.16
LG3	765	172	53.95	0.31	5.68
LG4	1 585	327	83.78	0.26	5.54
LG5	296	43	23.60	0.55	6.78
LG6	6 311	1 085	63.71	0.06	1.83
LG7	220	47	45.26	0.96	12.20
LG8	233	57	40.06	0.70	12.23
LG9	81	17	22.40	1.32	9.41
LG10	103
LG11	1 289	302	114.77	0.38	7.46
LG12	679	157	43.38	0.28	3.57
LG13	347	67	24.26	0.36	4.19
LG14	236	50	26.07	0.52	7.46
LG15	414	128	68.28	0.53	8.37
LG16	396	89	27.28	0.31	4.09
LG17	132	11	2.64	0.24	0.70
LG18	162	33	30.46	0.92	12.06
总计 Total	10 740	3 418	864.61

染色体 Chromosome	SNP 标记数量 No. of SNP marker	bin 标记数量 No. of bin marker	连锁群长度/cM Linkage distance	平均遗传距离/cM Average inter-loci distance	图谱空隙/cM Map gap
LG1	516	126	75.56	0.60	10.59
LG2	3 275	707	119.16	0.17	7.16
LG3	765	172	53.95	0.31	5.68
LG4	1 585	327	83.78	0.26	5.54
LG5	296	43	23.60	0.55	6.78
LG6	6 311	1 085	63.71	0.06	1.83
LG7	220	47	45.26	0.96	12.20
LG8	233	57	40.06	0.70	12.23
LG9	81	17	22.40	1.32	9.41
LG10	103
LG11	1 289	302	114.77	0.38	7.46
LG12	679	157	43.38	0.28	3.57
LG13	347	67	24.26	0.36	4.19
LG14	236	50	26.07	0.52	7.46
LG15	414	128	68.28	0.53	8.37
LG16	396	89	27.28	0.31	4.09
LG17	132	11	2.64	0.24	0.70
LG18	162	33	30.46	0.92	12.06
总计 Total	10 740	3 418	864.61

染色体 Chromosome	基因编号 Gene ID	基因功能注释 Annotation
6	Hca.06G0027130	MYB6转录因子
6	Hca.06G0027530	蔗糖转运蛋白
6	Hca.06G0027640	E3泛素蛋白连接酶
6	Hca.06G0027830	葡萄糖-6-磷酸脱氢酶
6	Hca.06G0028110	生长素相关蛋白
6	Hca.06G0028380	bHLH6转录因子
6	Hca.06G0028430	丝氨酸/苏氨酸蛋白激酶

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