外源2,4-表油菜素内酯对NaCl胁迫下燕麦幼苗光合特性的影响

doi:10.7668/hbnxb.20190663

摘要/Abstract

摘要： 为探明外源2，4-表油菜素内酯（EBR）对NaCl胁迫下燕麦光合作用的调控机理，以加燕2号、青引2号燕麦为材料，在100 mmol/L NaCl胁迫下，研究外源EBR对燕麦幼苗光合气体交换参数、荧光动力学参数、光合电子传递速率及PSⅡ光能分配情况的影响。结果表明：NaCl胁迫显著降低了燕麦幼苗的光合色素含量，抑制了PSⅡ反应中心活性，导致燕麦幼苗PSⅡ光化学效率下降，净光合速率（Pn）显著降低。和NaCl胁迫相比较，添加外源EBR后，燕麦幼苗叶片的叶绿素a（Chla）、叶绿素b（Chlb）和类胡萝卜素（Chlx·c）含量显著增加；燕麦幼苗的Pn、蒸腾速率（Tr）、气孔导度（Gs）和水分利用效率（WUE）显著升高，胞间CO₂浓度（Ci）显著降低，最大荧光（Fm）、光化学淬灭系数（qP）、PSⅡ实际光合效率（ФPSⅡ）、PSⅡ潜在活性（Fv/Fo）、PSⅡ原初光能转化效率（Fv/Fm）、天线色素转化效率（Fv'/Fm'）、表观光合电子传递速率（ETR）及PSⅡ光化学反应能（P）显著升高，初始荧光（Fo）、非光化学淬灭系数（NPQ）及反应中心耗散能（E）、天线耗散能（D）显著降低。综上所述，外源EBR能够显著提高NaCl胁迫下燕麦幼苗PSⅡ反应中心的实际光化学效率，有效缓解NaCl胁迫对燕麦幼苗所产生的光抑制和PSⅡ反应中心损伤程度，提高燕麦幼苗的碳同化效率和光合能力，说明外源BRs对盐胁迫下燕麦光合作用起到正向调控作用，能够有效缓解盐胁迫对燕麦幼苗所造成的伤害。

关键词: 燕麦, NaCl胁迫, 2,4-表油菜素内酯, 光合作用

Abstract: To understand the regulation mechanism of exogenous 2,4-epibrassinolide (EBR) on photosynthesis of oats under NaCl stress, the effects of exogenous EBR on photosynthetic gas exchange parameters, fluorescence kinetic parameters, photosynthetic electron transfer rate and PSⅡ light energy distribution of oat seedlings were studied under 100 mmol/L NaCl stress with Avena sativa L. cv. Jiayan 2 and Qingyin 2 as experimental materials. The results showed that, NaCl stress significantly reduced the photosynthetic pigment content of oat seedlings, inhibited the activity of PSⅡ reaction center, and led to the decrease of PSⅡ photochemical efficiency and Net photosynthetic rate (Pn) of oat seedlings. Compared with NaCl stress, exogenous EBR treatments significantly increased the contents of chlorophyll a (Chla), chlorophyll b (Chlb) and carotenoids in the leaves of oat seedlings; significantly increased photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs) and water use efficiency (WUE),but decreased intercellular CO₂ concentration (Ci); exogenous EBR treatments also significantly increased maximum fluorescence (Fm), photochemical quenching (qP), photochemical quantum yield of photosystem II (ФPS Ⅱ), potential photochemical efficiency (Fv/Fo),maximal chemical efficiency (Fv/Fm), conversion efficiency of antenna pigment (Fv'/Fm'), photosynthetic electron transport rate (ETR), PSⅡ photochemical reaction energy (P), but reduced initial fluorescence (Fo) and non photochemical quenching coefficient (NPQ), antenna heat dissipation (D) and reaction center dissipation (E). In conclusion, exogenous EBR could significantly improve the actual photochemical efficiency of PSⅡ reaction center of oat seedlings under NaCl stress, effectively alleviate the photoinhibition and the degree of damage in the PSⅡ reaction center induced by NaCl stress, and improve the carbon assimilation efficiency and photosynthetic capacity of oat seedlings. These results indicated that exogenous BRs played a positive role in the regulation of the photosynthesis under salt stress, which could alleviate the damage caused by salt stress on oat seedlings.

Key words: Avena sativa L., NaCl stress, 2,4-epibrassinolide, Photosynthesis

中图分类号:

S634.01

寇江涛. 外源2,4-表油菜素内酯对NaCl胁迫下燕麦幼苗光合特性的影响[J]. 华北农学报, 2020, 35(2): 79-87. doi: 10.7668/hbnxb.20190663.

KOU Jiangtao. Effects of Exogenous 2,4-epibrassinolide on Photosynthetic Characteristics of Oat Seedlings under NaCl Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(2): 79-87. doi: 10.7668/hbnxb.20190663.

http://www.hbnxb.net/CN/Y2020/V35/I2/79

参考文献

[1] 刘建新, 欧晓彬, 王金成. 镧胁迫下外源H₂O₂对裸燕麦幼苗叶绿素荧光参数和光合碳同化酶活性的影响[J].生态学报, 2019, 39(8):2833-2841. doi:10.5846/stxb201804200902.
Liu J X, Ou X B, Wang J C. Effects of exogenous hydrogen peroxide on chlorophyll fluorescence parameters and photosynthetic carbon assimilation enzymes activities in naked oat seedlings under lanthanum stress[J]. Acta Ecologica Sinica, 2019, 39(8):2833-2841.
[2] 厉书豪, 李曼, 张文东, 李仪曼, 艾希珍, 刘彬彬, 李清明. CO₂加富对盐胁迫下黄瓜幼苗叶片光合特性及活性氧代谢的影响[J]. 生态学报, 2019, 39(6):2122-2130. doi:10.5846/stxb201712212296.
Li S H, Li M, Zhang W D, Li Y M, Ai X Z, Liu B B, Li Q M. Effects of CO₂enrichment on photosynthetic characteristics and reactive oxygen species metabolism in leaves of cucumber seedlings under salt stress[J]. Acta Ecologica Sinica, 2019, 39(6):2122-2130.
[3] 韩静, 李旭芬, 石玉, 李艳琪, 韩瑞锋, 张毅. 盐胁迫下外源Si和Spd对番茄幼苗生长及光合荧光特性的影响[J]. 东北农业大学学报, 2019, 50(11):17-23,70. doi:10.19720/j.cnki.issn.1005-9369.2019.11.0003.
Han J, Li X F, Shi Y, Li Y Q, Han R F, Zhang Y. Effect of exogenous silicon and spermidine on growth and photosynthetic fluorescence characteristics of tomato seedlings under salt stress[J]. Journal of Northeast Agricultural University, 2019, 50(11):17-23,70.
[4] Chaves M M, Flexas J, Pinheiro C. Photosynthesis under drought and salt stress:regulation mechanisms from whole plant to cell[J]. Annals of Botany, 2009, 103(4):551-560. doi:10. 1093/aob/mcn125.
[5] 寇江涛, 师尚礼. 2,4-表油菜素内酯对NaCl胁迫下紫花苜蓿幼苗根系生长抑制及氧化损伤的缓解效应[J]. 中国生态农业学报, 2015, 23(8):1010-1019. doi:10.13930/j.cnki.cjea.150411.
Kou J T, Shi S L. 2,4-Epibrassinolide protection aginest root growth inhibition and oxidative damage of Medicago sativa L. seedling under NaCl stress[J]. Chinese Journal of Eco-Agriculture, 2015, 23(8):1010-1019.
[6] 王传凯, 郭淼. 油菜素内酯对裸燕麦常温和高温胁迫下生长形态及生理特性的影响[J]. 南方农业学报, 2017, 48(7):1173-1177. doi:10.3969/j.issn.2095-1191.2017.07.07.
Wang C K, Guo M. Effects of brassinolide on growth morphology and physiological characters of Avena nuda under room and high temperature stresses[J]. Journal of Southern Agriculture, 2017, 48(7):1173-1177.
[7] 杨慧杰, 原向阳, 郭平毅, 董淑琦, 张丽光, 温银元, 宋喜娥, 王宏富. 油菜素内酯对阔世玛胁迫下谷子叶片光合荧光特性及糖代谢的影响[J]. 中国农业科学, 2017, 50(13):2508-2518. doi:10.3864/j.issn.0578-1752.2017.13.010.
Yang H J, Yuan X Y, Guo P Y, Dong S Q, Zhang L G, Wen Y Y, Song X E, Wang H F. Effects of brassinolide on photosynthesis, chlorophyll fluorescence characteristics and carbohydrates metabolism in leaves of foxtail millet (Setaria italica) under sigma broad stress[J]. Scientia Agricultura Sinica, 2017, 50(13):2508-2518.
[8] 孙彤彤, 武春成, 宋士清. 外源水杨酸(SA)、油菜素内酯(BR)浸种对Ca(NO₃)₂胁迫下黄瓜幼苗光合特性及叶片解剖结构的影响[J]. 江苏农业学报, 2019, 35(5):1184-1190. doi:10.3969/j.issn.1000-4440.2019.05.026.
Sun T T, Wu C C, Song S Q. Effects of exogenous salicylic acid(SA) and brassinosteroids(BR) soaking on photosynthetic characteristics and leaf anatomical structure of cucumber seedlings under Ca(NO₃)₂ stress[J]. Jiangsu Journal of Agricultural Sciences, 2019, 35(5):1184-1190.
[9] 魏湜, 吉彪, 李赞, 顾万荣. 盐胁迫下油菜素内酯对玉米幼苗生理特性的影响[J]. 东北农业大学学报, 2018, 49(5):9-16. doi:10.3969/j.issn.1005-9369.2018.05.002.
Wei T, Ji B, Li Z, Gu W R. Effect of brassinolide on physiological characteristics of maize seedlings under salt stress[J]. Journal of Northeast Agricultural University, 2018, 49(5):9-16.
[10] 吴秀, 陆晓民. 亚适宜温光盐环境下油菜素内酯对黄瓜幼苗抗氧化系统及光合作用的影响[J]. 应用生态学报, 2015, 26(9):2751-2757. doi:10.13287/j.1001-9332.20150630.010.
Wu X, Lu X M. Effects of brassinolide on the antioxidant system and photosynthesis of cucumber seedlings under suboptimal temperature,light and salt environment[J]. Chinese Journal of Applied Ecology, 2015, 26(9):2751-2757.
[11] 马梅, 刘冉, 郑春芳, 刘伟成, 尹晓明, 刘金隆, 王长海, 郑青松. 油菜素内酯对盐渍下油菜幼苗生长的调控效应及其生理机制[J]. 生态学报, 2015, 35(6):1837-1844. doi:10.5846/stxb201305231157.
Ma M, Liu R, Zheng C F, Liu W C, Yin X M, Liu J L, Wang C H, Zheng Q S. Regulation of exogenous brassinosteroid on growth of salt-stressed canola seedlings and its physiological mechanism[J]. Acta Ecologica Sinica, 2015, 35(6):1837-1844.
[12] 孙仁国, 赵桂琴, 胡凯军, 满元荣. 盐胁迫对燕麦地上干物质积累及灌浆期光合特性的影响[J]. 中国草地学报, 2010, 32(5):15-20.
Sun R G, Zhao G Q, Hu K J, Man Y R. Effect of salinity stress on aboveground dry matter accumulation of oat and photosynthesis at grain filling stage[J]. Chinese Journal of Grassland, 2010, 32(5):15-20.
[13] 武俊英, 刘景辉, 李倩. 盐胁迫对燕麦幼苗生长, K⁺、Na⁺吸收和光合性能的影响[J]. 西北农业学报, 2010, 19(2):100-105. doi:10.3969/j.issn.1004-1389.2010.02.021.
Wu J Y, Liu J H, Li Q. Effects of salt stress on oat seedling growth and selective absorption of K⁺ and Na⁺ and photosynthetic characters[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2010, 19(2):100-105.
[14] 刘建新, 王金成, 王瑞娟, 刘秀丽. 混合盐碱胁迫对燕麦幼苗矿质离子吸收和光合特性的影响[J]. 干旱地区农业研究, 2017, 35(1):178-184. doi:10.7606/j.issn.1000-7601.2017.01.27.
Liu J X, Wang J C, Wang R J, Liu X L. Effect of complex saline-alkali stress on the mineral ions absorption and photosynthetic characteristics of oat seedlings[J]. Agricultural Research in the Arid Areas, 2017, 35(1):178-184.
[15] 寇江涛. 外源2,4-表油菜素内酯对NaCl胁迫下燕麦种子萌发和生理的影响[J]. 草地学报, 2019, 27(6):1562-1568. doi:10.11733/j.issn.1007-0435.2019.06.013.
Kou J T. Effects of 2,4-epibrassinolide on germination and physiological characteristics of Avena sativa L. seeds under NaCl stress[J]. Acta Agrestia Sinica, 2019, 27(6):1562-1568.
[16] Arnon D I. Copper enzymes in isolated chloroplasts:polyphenol oxidase in Beta vulgaris[J]. Plant Physiology, 1949, 24:1-15. doi:10.1104/pp.24.1.1.
[17] 寇江涛, 康文娟, 苗阳阳, 师尚礼. 外源2,4-表油菜素内酯对NaCl胁迫下紫花苜蓿幼苗光合特性及离子吸收、运输和分配的影响[J]. 草业学报, 2016, 25(4):91-103. doi:10.11686/cyxb2015289.
Kou J T, Kang W J, Miao Y Y, Shi S L. Effect of exogenous 2,4-epibrassinolide on the uptake, transport, and disputation of ions, and photosynthetic characteristics of Medicago sativa seedlings under NaCl stress[J]. Acta Prataculturae Sinica, 2016, 25(4):91-103.
[18] 寇江涛, 康文娟, 苗阳阳, 师尚礼. 外源EBR对NaCl胁迫下紫花苜蓿幼苗微量元素吸收及叶绿素荧光动力学参数的影响[J]. 中国生态农业学报, 2016, 24(3):345-355. doi:10.13930/j.cnki.cjea.151028.
Kou J T, Kang W J, Miao Y Y, Shi S L. Effect of exogenous 2,4-epibrassinolide on trace element absorption and chlorophyll fluorescence of Medicago sativa under NaCl stress[J]. Chinese Journal of Eco-Agriculture, 2016, 24(3):345-355.
[19] 胡文海, 胡雪华, 邹桂花, 曾庆梅, 吴琴玉. 高温干旱对2个辣椒品种PSⅡ功能与光能分配的影响[J]. 江西农业大学学报, 2010, 32(4):695-699. doi:10.3969/j.issn.1000-2286.2010.04.012.
Hu W H, Hu X H, Zou G H, Zeng Q M, Wu Q Y. Effects of heat and drought stresses on the PSⅡ functions and absorbed light allocation in two pepper (Capsicum annuum L.) cultivars[J]. Acta Agriculturae Universitatis Jiangxiensis, 2010, 32(4):695-699.
[20] 苏兰茜, 白亭玉, 鱼欢, 吴刚, 谭乐和. 盐胁迫对2种菠萝蜜属植物幼苗生长及光合荧光特性的影响[J]. 中国农业科学, 2019, 52(12):2140-2150. doi:10.3864/j.issn.0578-1752.2019.12.011.
Su L X, Bai T Y, Yu H, Wu G, Tan L H. Effects of salt stress on seedlings growth, photosynthesis and chlorophyll fluorescence of two secies of Artocarpus[J]. Scientia Agricultura Sinica, 2019, 52(12):2140-2150.
[21] 杨柳燕,陈菁菁,陈年来. 甜瓜叶片光合产物输出能力对弱光的响应[J]. 中国农业科学, 2018, 51(13):2561-2569. doi:10.3864/j.issn.0578-1752.2018.13.011.
Yang L Y, Chen J J, Chen N L. Responses of leaf assimilate export to lowlight stress in melon[J]. Scientia Agricultura Sinica, 2018, 51(13):2561-2569.
[22] Merzlyak M N, Solovchenko A E. Photostability of pigments in ripening apple fruit:a possible photoprotective role of carotenoids during plant senescence[J]. Plant Science, 2002, 163(4):881-888. doi:10.1016/s0168-9452(02)00241-8.
[23] 杨东清, 董文华, 骆永丽, 宋文挺, 蔡铁, 李勇, 尹燕枰, 王振林. 外源6-BA对两种氮素水平下小麦幼苗叶片光合性能及内源激素含量的影响[J]. 中国农业科学, 2017, 50(20):3871-3884. doi:10.3864/j.issn.0578-1752.2017.20.004.
Yang D Q, Dong W H, Luo Y L, Song W T, Cai T, Li Y, Yin Y P, Wang Z L. Effects of exogenous 6-BA on photosynthetic characteristics and endogenous hormone content in wheat leaves under two nitrogen application levels at seedling stage[J]. Scientia Agricultura Sinica, 2017, 50(20):3871-3884.
[24] 李涛涛, 高永峰, 马瑄, 陈永富, 王阳, 马金彪. 外源油菜素内酯对三种杨树在干旱、盐和铜胁迫下光合生理的影响[J]. 基因组学与应用生物学, 2016, 35(1):218-226. doi:10.13417/j.gab.035.000218.
Li T T, Gao Y F, Ma X, Chen Y F, Wang Y, Ma J B. Effects of exogenous brassinosteroid on photosynthesis of three spesies of Populus under drought, salt and copper stress[J]. Genomics and Applied Biology, 2016, 35(1), 218-226.
[25] 李彩凤, 刘丹, 邹春雷, 刘磊,武沛然,杨芳芳,王彬,王玉波. 盐碱胁迫下喷施BR对甜菜光系统Ⅱ和氮代谢关键酶的影响[J]. 东北农业大学学报, 2018, 49(8):39-47. doi:10.3969/j.issn.1005-9369.2018.08.005.
Li C F, Liu D, Zou C L, Liu L, Wu P R, Yang F F, Wang B, Wang Y B. Effect of spraying brassinolide (BR) on PSⅡ and key enzymes of nitrogen metabolism in sugar beet under saline-alkali stress[J]. Journal of Northeast Agricultural University, 2018, 49(8):39-47.
[26] 尹博, 梁国鹏, 贾文, 崔秀敏. 外源油菜素内酯介导Cu胁迫下番茄生长及Cu、Fe、Zn的吸收与分配[J]. 中国生态农业学报, 2014, 22(5):578-584. doi:10.3724/SP.J.1011.2014.31245.
Yin B, Liang G P, Jia W, Cui X M. Exogenous EBR mediated the plant growth and absorption and accumulation of Cu, Fe and Zn in tomato seedlings under Cu stress[J]. Chinese Journal of Eco-Agriculture, 2014, 22(5):578-584.
[27] Farquhar G D, Sharkey T D. Stomatal conductance and photosynthesis[J]. Annual Review of Plant Physiology, 1982, 33:317-345. doi:10.1146/annurev.pp.33.060182.001533.
[28] 吴雪霞, 查丁石. NaCl胁迫对茄子幼苗叶片叶绿素荧光参数和能量分配的影响[J]. 华北农学报, 2009, 24(6):83-87. doi:10.7668/hbnxb.2009.06.017.
Wu X X, Zha D S. Effects of NaCl stress on chlorophyll fluorescence parameters and excitation energy dissipation in leaves of egg plant seedlings[J]. Acta Agriculturae Boreali-Sinica, 2009, 24(6):83-87.
[29] 张永平, 杨少军, 陈幼源. 2,4-表油菜素内酯对高温胁迫下甜瓜幼苗抗氧化酶活性和光合作用的影响[J]. 西北植物学报, 2011, 31(7):1347-1354.
Zhang Y P, Yang S J, Chen Y Y. Effects of 2,4-epibrassinolide on antioxidant enzyme activities and photosynthesis in melon seedlings under high temperature stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(7):1347-1354.
[30] 李宁, 王美月, 孙锦, 束胜, 郭世荣, 王军伟, 孙洪助. 外源24-表油菜素内酯对弱光胁迫下番茄幼苗生长及光合作用的影响[J]. 西北植物学报, 2013, 33(7):1395-1402.
Li N, Wang M Y, Sun J, Shu S, Guo S R, Wang J W, Sun H Z. Effects of exogenous 24-epibrassinolide on growth and photosynthesis of tomato seedlings under low light stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2013, 33(7):1395-1402.
[31] 李蒙, 刘松虎, 张琰, 岳建华, 任毛飞. 外源24-表油菜素内酯对弱光胁迫下黄心菜光合特性和品质的影响[J]. 北方园艺, 2019, 43(10):72-79. doi:10.11937/bfyy.20183249.
Li M, Liu S H, Zhang Y, Yue J H, Ren M F. Effects of exogenous 24-epibrassinolide on photosynthetic characteristics and quality of Brassica pekinensis under weak light stress[J]. Northern Horticulture, 2019, 43(10):72-79.
[32] 李蒙, 范高领, 张燕, 刘松虎, 张琰. 外源2,4-表油菜素内酯(EBR)对弱光胁迫下黄心菜生长和碳水化合物代谢的影响[J]. 南方农业学报, 2019, 50(5):1028-1034. doi:10.3969/j.issn.2095-1191.2019.05.16.
Li M, Fan G L, Zhang Y, Liu S H, Zhang Y. Effects of exogenous 2,4-epibrassinolide(EBR)on growth and carbohydrate metabolism of Brassica pekinensis Lour. Rupr. under weak light stress[J]. Journal of Southern Agriculture, 2019, 50(5):1028-1034.
[33] 王可玢, 许春辉, 赵福洪, 唐崇钦, 戴云玲. 水分胁迫对小麦旗叶某些体内叶绿素a荧光参数的影响[J]. 生物物理学报, 1997, 13(2):273-278.
Wang K B, Xu C H, Zhao F H, Tang C Q, Dai Y L. The effects of water stress on some in vivo chlorophyll a fluorescence parameters of wheat flag leaves[J]. Acta Biophysica Sinica, 1997, 13(2):273-278.
[34] 寇江涛, 师尚礼, 胡桂馨, 周万海. 紫花苜蓿对牛角花齿蓟马为害的光合生理响应[J]. 生态学报, 2014, 34(20):5782-5792. doi:10.5846/stxb201301250157.
Kou J T, Shi S L, Hu G X, Zhou W H. Photosynthetic physiological response of Medicago sativa to Odontothrips loti damage[J]. Acta Ecologica Sinica, 2014, 34(20):5782-5792.
[35] Lichtenthaler H K. Chlorophylls and carotenoids:pigments of photosynthetic biomembranes[J]. Methods in Enzymology, 1987, 148:350-382. doi:10.1016/0076-6879(87)48036-1.
[36] Genty B, Briantais J M, Baker N R. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J]. Biochimica et Biophysica Acta, 1989, 990(1):87-92. doi:10.1016/s0304-4165(89)80016-9.
[37] Karuse G H, Weis E. Chlorophyll fluorescence as a tool in plant physiology:Ⅱ. Interpretation of fluorescence signals[J]. Photosynthesis Research, 1984, 5(2):139-157. doi:10.1007/BF00028527.
[38] 李淑叶, 马慧娟, 张思平, 刘绍东, 沈倩, 陈静, 葛常伟, 庞朝友, 赵新华. 外源24-表油菜素内酯对低温胁迫下棉花幼苗光合生理的影响[J]. 棉花学报, 2018, 30(3):252-260. doi:10.11963/1002-7807.
lsyzxh.20180409.
Li S Y, Ma H J, Zhang S P, Liu S D, Shen Q, Chen J, Ge C W, Pang C Y, Zhao X H. Effects of exogenous 24-epibrassinolide on photosynthetic physiology of cotton-seedlings under low temperature[J]. Cotton Science, 2018, 30(3):252-260.
[39] Bilger W, Bjorkman O. Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis[J]. Photosynthesis Research, 1990, 25(3):173-185. doi:10.1007/bf00033159.
[40] 杨万基, 蒋欣梅, 高欢, 刘舒娅, 孙天宇, 马创举, 白国梁, 于锡宏. 28-高芸苔素内酯对低温弱光胁迫辣椒幼苗光合和荧光特性的影响[J]. 南方农业学报, 2018, 49(4):741-747. doi:10.3969/j.issn.2095-1191.2018.04.19.
Yang W J, Jiang X M, Gao H, Liu S Y, Sun T Y, Ma C J, Bai G L, Yu X H. Effects of 28-homobrassinolide on photosynthetic and fluorescence characteristics of pepper seedlings under low temperature with dim light[J]. Journal of Southern Agriculture, 2018, 49(4):741-747.
[41] Wise R R, Naylor A W. Chilling-enhanced photoxidation:evidence for the role of singlet oxygen and superoxide in the breakdown of pigments and endogenous antioxidants[J]. Plant Physiology, 1987, 83(2):278-282. doi:10.1104/pp.83.2.278.
[42] Li J, Yang P, Gan Y T, Yu J H, Xie J M. Brassinosteroid alleviates chilling-induced oxidative stress in pepper by enhancing antioxidation systems and maintenance of photosystem Ⅱ[J]. Acta Physiologiae Plantarum, 2015, 37(11):222-233. doi:10.1007/s11738-015-1966-9.

选择文件类型/文献管理软件名称

选择包含的内容