高通量测序技术对早熟棉中miRNA的鉴定与分析

doi:10.7668/hbnxb.20190698

摘要/Abstract

摘要： 棉花中miRNA的数量仍较少，miRNA在棉花早熟发育中的作用有待证明和揭示。为丰富棉花中miRNA的数量，同时，为揭示miRNA在早熟棉发育中的作用，以早熟棉花品种石早1和中熟棉花品种冀棉958为材料，利用高通量测序技术构建了2个小RNA文库，鉴定新的miRNA，比较早熟棉花和中熟棉花中miRNA表达的差异、特点。结果共鉴定到131个miRNA，包括41个不同家族的64个已知miRNA和67个新的miRNA；新miRNA中，54个miRNA属于已知miRNA家族，13个miRNA属于新的miRNA家族。比较2个品种中miRNA表达的差异，共发现39个miRNA在2个品种中的表达差异超过2倍，占总miRNA数的29.8%，主要为中低量表达的miRNA。15个高表达miRNA中，只有miR398家族的ghr_21在2个品种中的表达差异超过2倍（达到3.4倍）；比较相同家族的不同miRNA在两品种中的表达差异，发现相同家族的miRNA在2个品种中表达差异可以不同，推测有不同的生物学功能。揭示了miRNA在早熟棉发育过程中，对其早熟性具有重要作用，为进一步研究miRNA在棉花早熟性调控中的作用提供依据。

关键词: 棉花, miRNA, 高通量测序, 早熟

Abstract: The amount of miRNA identified in cotton is still limited and the role of miRNAs in early-maturing cotton remains to be proved and revealed. In order to enrich the amount of miRNA in cotton and reveal the important roles of miRNA in the development of early-maturing cotton, two small RNA libraries were constructed using Shizao 1, a early-maturing cotton variety and Jimian 958, a medium cotton variety by high-throughput sequencing technology. As a result, a total of 131 miRNAs were identified, including 64 known miRNAs belonging to 41 miRNA family and 67 novel miRNAs; 54 of the 67 novel miRNAs belonged to known miRNA family and 13 novel miRNAs belonged to novel miRNA family. The expression of miRNAs were compared in the two cotton varieties, it found that 39 miRNAs showed obviously different expression(>2 fold) between the two varieties, which accounted for 29.8% and focused on low- and mid-expressed miRNAs. In the top 15 highly expressed miRNAs, only ghr_21(miR398 family) showed obviously different expression(3.4 fold). The expression of miRNAs belonging to the same family were compared and found different expression pattern in the two varieties. Above results revealed that miRNAs had important roles of earliness in the development of early-maturing cotton, which provide a basis for further study on the role of miRNA in the regulation of cotton prematurity.

Key words: Cotton, miRNA, High-throughput sequencing, Earliness

中图分类号:

S562.03

董章辉, 朱青竹, 赵丽芬, 眭书祥, 李增书, 张艳丽, 王虎, 郭永召, 姚则羊, 赵国忠. 高通量测序技术对早熟棉中miRNA的鉴定与分析[J]. 华北农学报, 2019, 34(S1): 15-21. doi: 10.7668/hbnxb.20190698.

DONG Zhanghui, ZHU Qingzhu, ZHAO Lifen, SUI Shuxiang, LI Zengshu, ZHANG Yanli, WANG Hu, GUO Yongzhao, YAO Zeyang, ZHAO Guozhong. Identification and Analysis of miRNAs in Early-maturing Cotton by High-Throughput Sequencing[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 15-21. doi: 10.7668/hbnxb.20190698.

http://www.hbnxb.net/CN/Y2019/V34/IS1/15

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