谷子SSR标记与光周期敏感性的关联分析

doi:10.7668/hbnxb.201750672

摘要/Abstract

摘要： 对光周期敏感是导致谷子生态适应性狭窄、生产上缺少跨区大品种的重要原因，鉴定谷子光周期敏感性相关联QTL位点是进一步揭示敏感形成遗传机制的基础。首先在长日照地区（河南省洛阳市）和短日照地区（海南省乐东县）对45份谷子品种进行抽穗期表型性状调查，并根据两地的抽穗期差异计算出光周期敏感值，最后进行SSR标记与光周期敏感性的关联分析。结果表明，40个SSR标记在45个谷子品种中共检测到150个等位基因，平均每个引物有3.75个等位基因，其中引物b200和b124的等位基因数目最多，均为6个。利用SSR标记对45份谷子材料进行群体遗传结构分析得到最佳K值为3，即将其划分为3个亚群：其中第1亚群有5个品种，第2亚群有16个品种，第3亚群有21个品种，而剩下的3个品种则不能划分到任何一组，成为混合群。连锁不平衡分析表明，40个SSR标记间不存在明显的连锁不平衡结构。SSR标记与光周期敏感性的关联分析检测到b200（SSR8）和b127（SSR35）2个位点与光周期敏感性关联（P<0.05）。

关键词: 谷子, 光周期敏感性, SSR标记, 关联分析

Abstract: Sensitivity to photoperiod is the main reason for narrow ecological adaptability and lacking cross-regional cultivars of foxtail millet. Identification of QTLs associated with millet photoperiod sensitivity is the foundation for further exploring genetic mechanisms of photoperiod sensitivity. 45 millet cultivars were surveyed for heading date in long-day region (Luoyang City, Henan Province) and short-day region (Ledong County, Hainan Province). The photoperiod sensitivity values of 45 millet cultivars were calculated according to the difference between the heading date in the two regions. Then, 40 pairs of SSR markers were used to determine the genotypes of these millet cultivars. Finally association analysis between SSR markers and photoperiod sensitivity was performed. The results showed that a total of 150 alleles were detected in 45 millet cultivars, giving an average allele number of 3.75 per locus. Of the 40 SSR markers, b200 and b124 amplified the most number of alleles (6 alleles per locus). Based on the SSR markers amplification data, the population genetic structure of 45 millet cultivars was analyzed, and the optimal K value was 3, so the 45 millet cultivars were divided into three subgroups. The first subgroup included 5 cultivars, the second subgroup included 16 cultivars and the third subgroup included 21 cultivars. The remaining three cultivars could not be grouped into any of the three subgroups, consisted of a mixed group. The linkage disequilibrium analysis showed that no significant linkage disequilibrium structures were found among 40 SSR markers. Finally association analysis between SSR markers and photoperiod sensitivity were performed, two loci, b200 (SSR8) and b127 (SSR35) were detected to associate with photoperiod sensitivity(P<0.05).

Key words: Millet, Photoperiod sensitivity, SSR markers, Association analysis

中图分类号:

S515.01

贾小平, 张博, 李剑峰, 袁玺垒, 陆平, 戴凌峰. 谷子SSR标记与光周期敏感性的关联分析[J]. 华北农学报, 2019, 34(1): 89-96. doi: 10.7668/hbnxb.201750672.

JIA Xiaoping, ZHANG Bo, LI Jianfeng, YUAN Xilei, LU Ping, DAI Lingfeng. Association Analysis of SSR Markers and Photoperiod Sensitivity in Foxtail Millet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 89-96. doi: 10.7668/hbnxb.201750672.

http://www.hbnxb.net/CN/Y2019/V34/I1/89

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