摘要： 为研究水稻SAND结构域蛋白的序列和功能,利用Blast在水稻基因组中搜索编码SAND结构域的基因,然后构建增强表达载体并通过遗传转化研究基因功能。结果表明,水稻基因LOC_Os01g57240(命名为OsULT1)编码的氨基酸序列含有SAND结构域和ULT B-box共有序列,与其他植物ULT氨基酸序列相似性为49.3%~92.3%,具有较好的保守性; 35S::OsULT1转基因株系部分颖花发育异常,出现内稃发育滞缓或退化、浆片过度发育或数目变多、雄蕊数目3~7枚、雌蕊2枚的现象;颖花内产生多余的小花,表明OsULT1对水稻花分生组织正常分化具有重要的调控作用。

关键词: 水稻, 花发育, SAND结构域, 基因, 序列

Abstract: To study the sequence and function of rice SAND domain protein, the gene encoding SAND was identified by Blast against the rice genome, and then over-expression vector was constructed to study its gene function by genetic transformation.The results found that the rice genome contains a gene LOC_Os01g57240 (named as OsULT1), which encoded amino acid sequence containing SAND domain and ULT B-box consensus sequence.The amino acid sequence similarity between OsULT1 and ULTs of other plants varied from 49.3% to 92.3%, they were highly conservative. The 35S::OsULT1 transgenic lines generated some mutant spikilets, which included under-developed or degenerated paleas, over-developed locidules, the increased number of locidules, the variant number of stamen, two pistils, or extra floret.The results indicates OsULT1 should play important roles in regulation of floral meristem identity in rice.

Key words: Rice(Oryza sativa L.), Floral development, SAND-domain, Gene, Sequence

中图分类号: 

• S511.03

• Q78