小麦转录因子基因<em>TaNF-YB2;1</em>表达特征及遗传转化对植株抵御干旱和盐分逆境能力的影响

doi:10.7668/hbnxb.2015.02.001

摘要/Abstract

摘要： NF-YB型转录因子在介导植物抵御非生物逆境中发挥着重要作用。为揭示小麦该家族成员 TaNF-YB2; 1 在干旱和盐分逆境下的表达特征及超表达 TaNF-YB2; 1 对植株抵御上述逆境能力的影响。利用半定量RT-PCR技术,研究 TaNF-YB2; 1 的表达特征,采用农杆菌介导的遗传转化技术,建立了超表达 TaNF-YB2; 1 的转基因烟草植株。结果表明,TaNF-YB2; 1 cDNA全长序列为958 bp,编码163个氨基酸残基。在24 h PEG模拟干旱和盐分处理条件下, TaNF-YB2; 1 的转录本数量明显上调。正常培养下,与野生型植株相比,转基因植株的生长和干物质积累量未发生改变;但在干旱和盐分处理下,转基因株系植株的生长较野生型植株明显改善,表现为植株个体体积增大,单株干物质积累量增多。因此, TaNF-YB2; 1 的表达受到干旱和盐分逆境的明显诱导,通过对干旱和盐分逆境应答,该基因在介导植株抵御上述环境逆境中发挥着重要功能。

关键词: 小麦, 转录因子基因 TaNF-YB2, 1, 表达, 旱盐胁迫, 抵御

Abstract: NF-YB type transcription factor family plays critical roles in mediating plant tolerance to diverse abiotic stresses.The purpose of this study was to understand the expression patterns of TaNF-YB2; 1, a NF-YB transcription factor gene in wheat, and determine the function of this gene in regulating plant tolerance to aforementioned stresses.The expression patterns of TaNF-YB2; 1 were determined based on semiquantitative RT-PCR and transgenic tobacco plants overexpressing TaNF-YB2; 1 were generated by adopting the Agrobacterium-mediated genetic transformation approach.The results indicated that TaNF-YB2; 1 had a cDNA full length of 958 bp, encoded a 163 amino acid-polypeptide.Under the conditions of drought and salt stress, the transcripts of TaNF-YB2; 1 were drastically upregualted in comparison those under the condition of normal growth, suggested that it responds to above stressors.Under normal growth, the growth features and dry mass of the plants overexpressing TaNF-YB2; 1 were similar to wild type.However, the plants overexpressing TaNF-YB2; 1 exhibited significantly improved growth features and dry mass compared with the wild type plants under treatments of drought and salt.Therefore, the expression of TaNF-YB2; 1 was dramatically induced by stresses of drought and high salinity. TaNF-YB2; 1 acts as a critical regulator in mediating plant tolerance to drought and salt through its response to above stressors.

Key words: Wheat, Transcription factor gene TaNF-YB2, 1, Expression, Drought and Salt stress, Tolerance

中图分类号:

S512.03
Q78

陈芳, 智一鸣, 肖凯. 小麦转录因子基因TaNF-YB2;1表达特征及遗传转化对植株抵御干旱和盐分逆境能力的影响[J]. 华北农学报, 2015, 30(2): 1-5. doi: 10.7668/hbnxb.2015.02.001.

CHEN Fang, ZHI Yi-ming, XIAO Kai. Expression Patterns of Wheat Transcription Factor Gene TaNF-YB2; 1 and Its Genetic Effects on Plant Tolerance to Stresses of Drought and Salt[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 1-5. doi: 10.7668/hbnxb.2015.02.001.

http://www.hbnxb.net/CN/Y2015/V30/I2/1

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小麦转录因子基因TaNF-YB2;1表达特征及遗传转化对植株抵御干旱和盐分逆境能力的影响

Expression Patterns of Wheat Transcription Factor Gene TaNF-YB2; 1 and Its Genetic Effects on Plant Tolerance to Stresses of Drought and Salt

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小麦转录因子基因TaNF-YB2;1表达特征及遗传转化对植株抵御干旱和盐分逆境能力的影响

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