摘要： 利用ISSR技术，对收集自5个国家的43份芦笋品种进行了遗传多样性分析，旨在为芦笋育种工作提供更多的参考数据。试验结果表明，18条引物共扩增条带159条，其中，多态性条带121条，多态性比率为76.10%。用软件PopGene 1.32 计算出供试芦笋的观察等位基因数为2，有效等位基因数为1.444 3，Nei's基因多样性指数为0.265 4，Shannon信息指数为0.411 4。 根据公式计算得出，多态性信息指数（PIC）为0.317~0.840，平均为0.669；基因组的平均遗传丰富度为5.17，表明供试芦笋具有较高的遗传多样性。利用NTSYS 2.10e软件进行相似系数分析，构建聚类树状图，在相似系数0.77处划线，可将样品划分为8个类群，其中，江西的JK104、西班牙的Pacific Peak、山东的082单独聚为一类，在其余分类中，美国与江西、美国与荷兰、荷兰与北京的一些品种间表现出较高的相似性。

关键词: 芦笋, ISSR, 聚类分析, 遗传多样性

Abstract: The genetic diversity of 43 asparagus cultivars collected from 5 countries was analyzed by ISSR method in order to provide more data reference for asparagus breeding.The results show that a total of 159 bands were amplified by PCR with 18 primers,among which 121 bands were polymorphic.The percentage of polymorphism was 76.10%.For these asparagus,the observed number of alleles was 2,the effective number of alleles was 1.444 3,the Nei's gene diversity was 0.265 4,and the Shannon's information index was 0.411 4.Calculated using the formula,the average polymorphism index content(PIC) was 0.669,ranged from 0.317 to 0.840.The genomic average genetic richness was 5.17.This indicates that the genetic diversity in the tested materials with high performance.The genetic similarity coefficient was analyzed,and a tree diagram was constructed by NTSYS 2.10e software.UPGMA cluster analysis showed that these cultivars could be categorized eight groups according to coefficient 0.77.The cultivars JK104 from Jiangxi,Pacific Peak from Spain and 082 from Shandong were respectively identified as different single group.In other group,the genetic similarity between the cultivars from American and Jiangxi,American and Holland,Holland and Beijing were relatively high.

Key words: Asparagus, ISSR, Cluster analysis, Genetic diversity

中图分类号: 

• S644.6