摘要： 利用磁珠富集法分离草金鱼的微卫星分子序列,获得38个阳性克隆,对其进行测序,获得31个微卫星DNA序列,利用软件Primer Premier5.0设计引物12对,其中有8对引物可扩增出稳定的目的条带。利用筛选出的8对微卫星引物对北京2个草金鱼养殖群体进行了遗传多样性分析。结果表明,2个草金鱼养殖群体的遗传多样性水平较高,其平均观测杂合度(Ho)分别为0.5655,0.5343,平均期望杂合度(He)分别为0.6570,0.6286,平均多态性信息含量PIC分别为0.5106,0.5009。两群体间遗传距离为0.0957,遗传相似度为0.9211。8个微卫星位点可用于金鱼群体的遗传学研究。

关键词: 金鱼, 微卫星, 磁珠富集, 遗传多样性

Abstract: Microsatellite DNA was isolated from goldfish with combining biotin capture method. We obtained 38 positive clones and isolated 31 microsatellite sequences. 12 pairs of primers were designed by Primer Premier 5.0 and 8 pairs of primers can obtain amplifying products among them. The genetic diversity of the artificial breed goldfish populations were studied by using the 8 Microsatellite primers. The results showed that the genetic diversity level of two populations were high. The average heterozygosity(Ho) in two populations was from 0. 534 3 to 0. 565 5,average excepted heterozygosity(He) was from 0. 628 6 to 0. 657 0,and average polymorphism information content (PIC) was from 0. 500 9 to 0. 510 6. The genetic distance was 0. 095 7 and genetic identity was 0.921 1.All of 8 microsatellite locus could be used for genetic analysis in goldfish.

Key words: Goldfish, Microsatellite, Enrichment by magnetic beads, Genetic diversity

中图分类号: 

• Q78