摘要： 为明确玉米群体的遗传变异性,利用SSR分子标记技术,采用12株叶片混合、每个群体5个混合样本提取DNA的最优取样方法,对12个玉米群体及6个对照自交系进行遗传多样性分析,试验筛选出86对SSR适宜引物,共扩增出391条多态性带,每个位点上的等位基因数为2～11条,平均5.67条,以GD值0.67为基准,划分为6个类群。蒙A群、蒙B群、中综5号、黄早4为第一类,蒙C群、蒙群1、掖478为第二类,蒙群2、蒙群4、C群1、C群2、Mo17为第三类,蒙群3、C群3、中综7号、B73为第四类,丹340和齐319各单独为一类,该结果与产量SCA效应分析划分结果基本一致。

关键词: 玉米群体, SSR标记, 遗传多样性

Abstract: The widely genetic diversity in maize population is the important basis for maize breeding. To clear the genetic basis and genetic diversity is very important for germplasm enhancement and improvement of maize. In this research,SSR molecular marker technology and the best sampling method,which DNA was extracted from five samples in each maize population and mixture of leaves of 12 plants, was adopted. The genetic diversity from 12 maize populations and 6 control inbred lines were analysed. 86 pairs SSR primers were filtered in this experiment, 391 polymorphic bands were gained. The number of alleles ranged from 2 to 11,the average is 5.76.The studied 12 maize populations and 6 control inbred lines could be partitioned into 6 class groups on the basis of 0. 67 for the GD value.Meng A,Meng B,Zhongzong 5 and Huangzao 4 are belonged to one group,Meng C,Mengqun 1 and Ye 478 are belonged to the second group,Mengqun 2,Mengqun 4,C Qun 1,C Qun 2 and Mo17 are belonged to the third group,Mengqun 3,C Qun 3,Zhongzong 7 and B73 are belonged to the fourth group,Dan 340 and Qi 319 are belonged to the fifth and sixth group,respectively. The partition is similar with the partition based on the yield SCA effect.

Key words: Maize population, SSR marker, Genetic diversity

中图分类号: 

• S513