不结球白菜品种基于SSR标记的群体结构分析

doi:10.3969/j.issn.1000-7091.2013.03.012

摘要/Abstract

摘要： 为了研究不结球白菜内部的群体结构,从全国搜集了78份广泛种植的蔬菜用不结球白菜品种,使用SSR标记对其进行多态性分析,并利用Structure 2.1软件统计分析标记数据划分群体结构。结果表明,初筛出的21对多态性SSR标记覆盖了白菜10个连锁群,在78份材料中扩增共产生了56个等位基因变异,平均每个SSR标记产生的等位基因数目为2.7个。Structure分析表明,所选的不结球白菜可分为4个亚类群,S1和S2亚类群主要集中了来自于南北方各省份的49份普通白菜,苗用大白菜主要分布在S3和S4亚类群,乌塌菜主要分布在S1和S4亚类群,菜心的遗传背景比较复杂;材料的类群划分与地理来源的关系在S2亚类群表现的较为明显。结果表明,不结球白菜内部存在着明显的群体结构,为合理利用现有的种质资源指导育种和进行新基因的挖掘奠定了基础。

关键词: 不结球白菜, SSR, 群体结构, 遗传多样性

Abstract: To study population structure within non-heading Chinese cabbage crops，78 non-heading Chinese cabbage varieties grown in a large planting area in China were collected． Their DNA polymorphisms were analyzed by a set of SSR markers and their population structure were clustered by Structure 2． 1 software according SSR data． Out of SSR primer 120 pairs， 21 pairs origining 10 linkage groups of Brassica rapa were screened out with polymorphism and 56 alleles in the 78 varieties were generated． The average number of alleles per SSR locus was 2． 7． The 56 markers were imported in the program structure to identify subpopulations， resulting in 4 subgroups in the 78 varieties．Subpopulations S1 and S2 mainly included 49 Pak choi varieties from different provinces in southern or northern China． Seedling Chinese cabbages were mainly distributed in subpopulation S3 and S4． Wutacai varieties were mainly distributed in subpopulation S1 and S4． The genetic backgrouds of Caixin varieties were relatively complicated． The relationship between material grouping and geographical origin was very clear in subpopulation S2． This result indicated an obvious population structure within non-heading Chinese cabbage，which established the basis for utilizing the current germplasm resources to guide breeding and discovering the new genes．

Key words: Non-heading Chinese cabbage, SSR, Population structure, DNA polymorphism

中图分类号:

S634.03

秦艳梅, 李向, 赵建军, 王彦华, 轩淑欣. 不结球白菜品种基于SSR标记的群体结构分析[J]. 华北农学报, 2013, 28(3): 62-66. doi: 10.3969/j.issn.1000-7091.2013.03.012.

Qin Yanmei, Li Xiang, Zhao Jianjun, Wang Yanhua, Xuan Shuxin. Analysis of Population Structure of Non-heading Chinese Cabbage Based on SSR Marker[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 62-66. doi: 10.3969/j.issn.1000-7091.2013.03.012.

http://www.hbnxb.net/CN/Y2013/V28/I3/62

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