摘要： 利用SSR标记技术对山西省12份马铃薯主栽品种进行了遗传多样性分析。从60对SSR引物中筛选出11对多态性高、谱带清晰的引物,共检测到79个等位位点,其中,77个多态性位点,多态性比率达97.5%;S189、S151和STM001这3个引物扩增的DNA指纹图谱差异明显,可作为12份马铃薯品种间鉴别的分子依据;12份材料的遗传距离介于0.209 2～0.626 9之间,平均值为0.421 7。在遗传相似系数为0.632处,将12份材料划分为4类,第Ⅰ类有3个品种(费乌瑞它、大西洋和冀张薯8号),同薯24和紫花白分别归为第Ⅲ类和第Ⅳ类,其余7个品种归为第Ⅱ类(其中全部为山西省选育的马铃薯品种,亲缘关系比较近)。结果说明,山西省现有马铃薯主栽品种遗传基础比较狭窄,种间差异不大。
关键词:
马铃薯,
主栽品种,
遗传多样性,
SSR分析,
聚类分析
Abstract: In this paper， the genetic diversities of 12 parts key potato cultivars in Shanxi province were analyzed by SSR markers． Eleven pairs of SSR primers with high polymorphism and clear bands were selected from 60 pairs of SSR primers． Among 79 SSR bands obtained from 11 primers， 77 bands were polymorphic accounting for 97． 5% of the total． The differences in SSR DNA fingerprints amplified with three primers S189，S151 and STM001 were significant． It could be used as molecular evidence to distinguish 12 potato varieties． The genetic distance of twelve potato cultivars ranged from 0． 209 2 to 0． 626 9，and the average value was 0． 421 7． At 0． 632 of genetic similarity， the 12 materials were clustered into 4 groups， the first group had three varieties( Feierruita，Atlantic and Jizhangshu-No． 8),Tongshu 24，Zihuabai were clustered into the third and the fourth groups separately． The other varieties， which bred in Shanxi province and showed a close genetic relationship，were clustered into the second group． Results showed that the genetic basis of the existing potato cultivars in Shanxi province is relatively narrow and the interspecies differences of these cultivars are not significant．
Key words:
Potato,
Main cultivars,
Genetic diversity,
SSR analysis,
Cluster analysis
中图分类号:
刘建霞, 雷海英, 温日宇, 郭耀东, 王润梅, 周凤. 山西省马铃薯主栽品种遗传多样性的SSR分析[J]. 华北农学报, 2012, 27(6): 72-77. doi: 10.3969/j.issn.1000-7091.2012.06.016.
LIU Jian-xia, LEI Hai-ying, WEN Ri-yu, GUO Yao-dong, WANG Run-mei, ZHOU Feng. SSR Analysis on Genetic Diversity of Leading Potato Cultivars in Shanxi Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 72-77. doi: 10.3969/j.issn.1000-7091.2012.06.016.