华北农学报 ›› 2012, Vol. 27 ›› Issue (6): 5-10. doi: 10.3969/j.issn.1000-7091.2012.06.002

所属专题: 油料作物

• 论文 • 上一篇    下一篇

大豆公共遗传图谱C1连锁群SSR标记空白区段的填补

雷雅坤1,2, 闫龙1, 杨春燕1, 宋晓昆1, 张孟臣1, 黄占景2   

  1. 1. 河北省农林科学院粮油作物研究所, 国家大豆改良中心石家庄分中心, 河北省遗传育种重点实验室, 河北石家庄 050031;
    2. 河北师范大学生命科学学院, 河北石家庄 050016
  • 收稿日期:2012-09-30 出版日期:2012-12-28
  • 通讯作者: 张孟臣(1956-),男,河北衡水人,研究员,硕士,主要从事大豆遗传育种研究;黄占景(1957-),男,河北深县人,教授,博士生导师,主要从事植物分子生物学研究。
  • 作者简介:雷雅坤(1985-),女,河北唐山人,硕士,主要从事植物生物化学与分子生物学研究。
  • 基金资助:
    国家"863"计划(2006AA10Z1B3;2012AA101106);国家自然科学基金(31000719);国家转基因生物新品种培育重大专项(2011ZX08009-088B);国家现代农业产业技术体系(CARS-004-PS06)

Complete the Blank Section with SSR Markers on Linkage Group C1 of Public Genetic Map in Soybean

LEI Ya-kun1,2, YAN Long1, YANG Chun-yan1, SONG Xiao-kun1, ZHANG Meng-chen1, HUANG Zhan-jing2   

  1. 1. Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang Branch Center of National Center for Soybean Improvement, North China Key Laboratory of Biology and Genetic Improvement of Soybean, Hebei Genetic Breeding Laboratory, Shijiazhuang 050031, China;
    2. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China
  • Received:2012-09-30 Published:2012-12-28

摘要: 为进一步饱和大豆公共图谱SSR标记,以大豆育成品种冀豆12×地方品种ZDD03651组合的211个F6株系为作图群体,以Kosambi作图函数构建SSR标记遗传连锁图谱。结果表明,栽培大豆冀豆12与大豆地方品种ZDD03651间SSR标记多态率为44.6%,遗传图谱包含21个连锁群,117个SSR标记,遗传距离总长度1 501 cM,标记间平均距离15.6 cM,其中包含8个偏分离标记。与公共遗传图谱相比,位点间排列顺序、遗传距离和偏分离位点比例基本相同。将SSR新标记Barcsoyssr41181、Barcsoyssr41201、Barcsoyssr41235和Barcsoyssr51266整合到C1连锁群上,填补了国际大豆公共遗传图谱中C1连锁群94.62~120.12 cM之间的SSR标记空白区段。

关键词: 地方品种, 遗传图谱, 偏分离, SSR

Abstract: Cross was made using bred varieties Jidou 12 × landrace soybean ZDD03651,get F6 RIL population with 211 single plants as the mapping population,construction of a SSR genetic linkage map with Kosambi mapping function, In order to saturate with SSR markers on public genetic map in the further. The polymorphic ratio was 44. 6% of SSR markers between Jidou 12 and ZDD03651. A total of 117 pairs of SSR markers on genetic linkage map, including 8 pairs distorted SSR markers,The resulting genetic linkage map covered 1 501 cM,with an average inter-marker distance of 15. 6 cM, including 21 linkage groups. Compared with public genetic map, the order between points,genetic distance and the proportion of distorted markers basically the same. And eventually the development of new molecular markers Barcsoyssr_4_1181,Barcsoyssr_4_1201,Barcsoyssr_4_1235,Barcsoyssr_5_1266 linked to the C1 linkage map, in order to complete the blank section on linkage group C1 between 94. 62 cM and 120. 12 cM in the international.

Key words: Landrace, Genetic mapping, Segregation distortion, SSR

中图分类号: 

引用本文

雷雅坤, 闫龙, 杨春燕, 宋晓昆, 张孟臣, 黄占景. 大豆公共遗传图谱C1连锁群SSR标记空白区段的填补[J]. 华北农学报, 2012, 27(6): 5-10. doi: 10.3969/j.issn.1000-7091.2012.06.002.

LEI Ya-kun, YAN Long, YANG Chun-yan, SONG Xiao-kun, ZHANG Meng-chen, HUANG Zhan-jing. Complete the Blank Section with SSR Markers on Linkage Group C1 of Public Genetic Map in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 5-10. doi: 10.3969/j.issn.1000-7091.2012.06.002.

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